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  • Publication
    Supplementary material from: Genetic characterization of the ibuprofen degradative pathway of Rhizorhabdus wittichii MPO218
    (American Society for Microbiology, 2022-04-19) Aulestia, Magaly; Flores, Amando; Acosta-Jurado, Sebastián; Santero, Eduardo; Camacho, Eva María
    Ibuprofen is one of the most common drugs found as contaminants in soils, sediments and waters. Although several microorganisms able to metabolize ibuprofen have been described, the metabolic pathways and factors limiting biodegradation in nature remain poorly characterized. Among the bacteria able to grow on ibuprofen, three different strains belonging to Sphingomonadaceae and isolated from different geographical locations, carry the same set of genes required for the upper part of the ibuprofen metabolic pathway. Here, we have studied the metabolic pathway of Rhizorhabdus wittichii MPO218, identifying new genes required for the lower part of the ibuprofen metabolic pathway. We have identified two new DNA regions in MPO218 involved in the metabolism of ibuprofen. One is located on the MPO218 chromosome and appears to be required for the metabolism of propionyl-CoA through the methylmalonyl-CoA pathway. Although involved in ibuprofen metabolism, this region is not strictly necessary for growing using ibuprofen. The second region belongs to the pIBU218 plasmid and comprises two gene clusters containing aromatic compounds biodegradation genes, part of which are necessary to ibuprofen degradation. We have identified two genes required for the first two steps of the lower part of the ibuprofen metabolic pathway (ipfL and ipfM) and, based on our results, we propose the putative complete pathway for ibuprofen metabolism in strain MPO218.
  • Publication
    Supplementary material 1 from: Luna A, Edelaar P, Shwartz A (2019) Assessment of social perception of an invasive parakeet using a novel visual survey method. NeoBiota 46: 71-89.
    (Pensoft Publishers, 2019-06-07) Luna, Álvaro; Edelaar, Pim; Shwartz, Assaf
    The perceptions of the general public regarding invasive alien species (IAS) are important in the prevention of future invasions and the success of management programmes. Here we use a novel visual method to investigate the perception of a charismatic IAS, the rose-ringed parakeet, across different stakeholders in Seville, Spain. Respondents were asked to select images of 10 bird species they would like to have present in their surroundings, out of 20 available images, including the parakeet and three other non-natives.
  • Publication
    Supplementary material 1 from: Strubbe D, White R, Edelaar P, Rahbek C, Shwartz A (2019) Advancing impact assessments of non-native species: strategies for strengthening the evidence-base. NeoBiota 51: 41-64.
    (Pensoft Publishers, 2019-11-06) Strubbe, Diederik; White, Rachel; Edelaar, Pim; Rahbek, Carsten; Shwartz, Assaf
    The numbers and impacts of non-native species (NNS) continue to grow. Multiple ranking protocols have been developed to identify and manage the most damaging species. However, existing protocols differ considerably in the type of impact they consider, the way evidence of impacts is included and scored, and in the way the precautionary principle is applied. These differences may lead to inconsistent impact assessments. Since these protocols are considered a main policy tool to promote mitigation efforts, such inconsistencies are undesirable, as they can affect our ability to reliably identify the most damaging NNS, and can erode public support for NNS management. Here we propose a broadly applicable framework for building a transparent NNS impact evidence base. First, we advise to separate the collection of evidence of impacts from the act of scoring the severity of these impacts. Second, we propose to map the collected evidence along a set of distinguishing criteria: where it is published, which methodological approach was used to obtain it, the relevance of the geographical area from which it originates, and the direction of the impact. This procedure produces a transparent and reproducible evidence base which can subsequently be used for different scoring protocols, and which should be made public. Finally, we argue that the precautionary principle should only be used at the risk management stage. Conditional upon the evidence presented in an impact assessment, decision-makers may use the precautionary principle for NNS management under scientific uncertainty regarding the likelihood and magnitude of NNS impacts. Our framework paves the way for an improved application of impact assessments protocols, reducing inconsistencies and ultimately enabling more effective NNS management.