García Daza, Fabián A.Rodríguez-Rivas, ÁlvaroGovantes, FernandoCuetos, Alejandro2025-07-112025-07-112025-07-07Colloids and Surfaces B: Biointerfaces, 255 (2025) 11490310.1016/j.colsurfb.2025.114903https://hdl.handle.net/10433/24415Proyectos de investigación PID2021-126121NB-I00The diffusion of particles in complex media has gained significant interest due to its dual relevance: probing the viscoelastic properties of materials via microrheology and assessing the extent of particle displacement over time. In this work, we explore the early-stage diffusion of a tracer particle within a developing bacterial biofilm using implicit-solvent Brownian dynamics simulations. At these initial stages, bacterial colonies form twodimensional structures that expand through cell growth and division. Employing an agent-based computational model (IbM), we analyze the passive diffusion of a spherical tracer within colonies of varying compaction levels. Our findings reveal that, at very short timescales, tracer diffusion follows a standard diffusive regime, modulated by colony aging. However, at longer times, the dominant factor governing tracer motion is colony growth, which effectively confines the tracer within the expanding structure, except in cases where the microcolony is highly unstructured or the tracer is sufficiently small. Additionally, through MR techniques, we quantify the elastic and viscous moduli of the growing microcolony, offering insight into its evolving viscoelastic behavior.application/pdfenAttribution-NonCommercial-ShareAlike 4.0 Internationalhttp://creativecommons.org/licenses/by-nc-sa/4.0/TracerDiffusionBiofilmMicrorheologyComputer simulation IBM modeljournal articleopen access