Fernández Abascal, JesúsChiaino, EldaFrosini, MariaDavey, Gavin PValoti, Massimo2025-01-282025-01-282020-05-31Fernandez-Abascal, J., Chiaino, E., Frosini, M., Davey, G. P., & Valoti, M. (2020). β-Naphthoflavone and Ethanol Reverse Mitochondrial Dysfunction in A Parkinsonian Model of Neurodegeneration. International Journal of Molecular Sciences, 21(11), 3955. https://doi.org/10.3390/ijms2111395510.3390/ijms21113955https://hdl.handle.net/10433/22761The 1-methyl-4-phenylpyridinium (MPP+) is a parkinsonian-inducing toxin that promotes neurodegeneration of dopaminergic cells by directly targeting complex I of mitochondria. Recently, it was reported that some Cytochrome P450 (CYP) isoforms, such as CYP 2D6 or 2E1, may be involved in the development of this neurodegenerative disease. In order to study a possible role for CYP induction in neurorepair, we designed an in vitro model where undifferentiated neuroblastoma SH-SY5Y cells were treated with the CYP inducers β-naphthoflavone (βNF) and ethanol (EtOH) before and during exposure to the parkinsonian neurotoxin, MPP+. The toxic effect of MPP+ in cell viability was rescued with both βNF and EtOH treatments. We also report that this was due to a decrease in reactive oxygen species (ROS) production, restoration of mitochondrial fusion kinetics, and mitochondrial membrane potential. These treatments also protected complex I activity against the inhibitory effects caused by MPP+, suggesting a possible neuroprotective role for CYP inducers. These results bring new insights into the possible role of CYP isoenzymes in xenobiotic clearance and central nervous system homeostasis.application/pdfenAttribution-NonCommercial-NoDerivatives 4.0 Internationalhttp://creativecommons.org/licenses/by-nc-nd/4.0/CYP 2D6CYP 2E1CYP inductionCytochrome P-450 systemMPP+ toxicityMitochondrial kineticsNeurodegenerationNeuroprotectionjournal articleopen access