RT Journal Article
T1 Isolation and genomic characterization of the ibuprofen-degrading bacterium Sphingomonas strain MPO218
A1 Aulestia, Magaly
A1 Flores, Amando
A1 Mangas Tena, Eugenio Luis
A1 Pérez-Pulido, Antonio J.
A1 Santero, Eduardo
A1 Camacho, Eva
K1 Genomic characterization
K1 Sphingomonas
AB The presence of pharmaceutical compounds in waters and soils is of particular concern because these compounds can be biologically active, even at environmental concentrations. Most pharmaceutical contaminants result from inefficient removal of these compounds during wastewater treatment. Although microorganisms able to biodegrade pharmaceuticals compounds have been described, the isolation and characterization of new bacterial strains capable of degrading drugs remain important to improve the removal of this pollutant. In this work, we describe the Sphingomonas wittichii strain MPO218 as able to use ibuprofen as the sole carbon and energy source. The genome of MPO218 consists of a circular chromosome and two circular plasmids. Our analysis shows that the largest plasmid, named pIBU218, is conjugative and can horizontally transfer the capability of growing on ibuprofen after conjugation with another related bacterium, Sphingopyxis granuli TFA. This plasmid appears to be unstable since it undergoes different deletions in absence of selection when growth on ibuprofen is not selected. This is the first described example of a natural and conjugative plasmid that enables growth on ibuprofen and is another example of how horizontal gene transfer plays a crucial role in the evolution of bacteria.
PB Wiley
YR 2021
FD 2021
LK https://hdl.handle.net/10433/25232
UL https://hdl.handle.net/10433/25232
LA en
NO Environ Microbiol . 2021 Jan;23(1):267-280. doi: 10.1111/1462-2920.15309. Epub 2020 Nov 27.
NO Spanish Ministry of Science, Innovation and Universi-ties grant BIO2014-57545-R
NO Universidad Pablo de Olavide. Departamento de Biología Molecular e Ingeniería Bioquímica
DS RIO
RD Apr 24, 2026