RT Journal Article
T1 Bioaccessibility and decomposition of cylindrospermopsin in vegetables matrices after the application of an in vitro digestion model
A1 Maisanaba, Sara
A1 Guzmán-Guillén, Remedios
A1 Valderrama, Rocío
A1 Meca, Giuseppe
A1 Font, Guillermina
A1 Jos, Ángeles
A1 Cameán, Ana M
K1 Cylindrospermopsin
K1 Bioaccessibility
K1 Decomposition products
K1 Lettuce
K1 Spinach
AB Research on the human exposure to Cylindrospermopsin (CYN) via consumption of contaminated food is of greatinterest for risk assessment purposes. The aim of this work is to evaluate for the first time the CYN bioaccessibilityin contaminated vegetables (uncooked lettuce and spinach, and boiled spinach) after an in vitro digestionmodel, including the salivar, gastric and duodenal phases and, colonic fermentation under lactic acid bacteria.The results obtained showed that the digestion processes are able to diminish CYN levels, mainly in the colonicphase, especially in combination with the boiling treatment, decreasing CYN levels in a significant way.Moreover, the potential decomposition products in a pure CYN solution and in CYN-contaminated vegetableswere evaluated using UHPLC-MS/MS Orbitrap. Under the conditions assayed, only two diastereoisomers of thesame fragment with m/z 292.09617 have been detected in all the analysed samples, with the exception ofdigested vegetables. Therefore, in terms of risk assessment, the digestion seems to play an important role inreducing the final bioaccesibility of CYN, and the consumption of cooked vegetables (spinach) would be safer incomparison to raw vegetables.
PB Elsevier
YR 2018
FD 2018-07-06
LK https://hdl.handle.net/10433/19731
UL https://hdl.handle.net/10433/19731
LA en
NO Food and Chemical Toxicology 120 (2018) 164–171
NO The authors would like to acknowledge the Ministerio de Economía y Competitividad of Spain (AGL2015-64558-R, MINECO/FEDER, UE) for its financial support.
NO Area of Toxicology, Faculty of Pharmacy, University of Sevilla
NO Laboratory of Food Chemistry and Toxicology, Faculty of Pharmacy, University of Valencia
DS RIO
RD May 9, 2026