RT Journal Article
T1 The fission yeast nucleoporin Alm1 is required for proteasomal degradation of kinetochore components.
A1 Salas-Pino, Silvia
A1 Gallardo Palomo, Paola
A1 Barrales, RR
A1 Braun, S
A1 Daga, Rafael
K1 Cell cycle and division
K1 Genetics
K1 Protein homeostasis
AB Kinetochores (KTs) are large multiprotein complexes that constitute the interface between centromeric chromatin and the mitotic spindle during chromosome segregation. In spite of their essential role, little is known about how centromeres and KTs are assembled and how their precise stoichiometry is regulated. In this study, we show that the nuclear pore basket component Alm1 is required to maintain both the proteasome and its anchor, Cut8, at the nuclear envelope, which in turn regulates proteostasis of certain inner KT components. Consistently, alm1-deleted cells show increased levels of KT proteins, including CENP-CCnp3, spindle assembly checkpoint activation, and chromosome segregation defects. Our data demonstrate a novel function of the nucleoporin Alm1 in proteasome localization required for KT homeostasis.
PB Rockefeller University Press
YR 2017
FD 2017-11-06
LK https://hdl.handle.net/10433/25244
UL https://hdl.handle.net/10433/25244
LA en
NO Cell Biol. 2017 Nov 6;216(11):3591-3608.
NO Departamento de Biología Molecular e Ingeniería Bioquímica. Centro Andaluz de Biología del Desarrollo
DS RIO
RD Apr 23, 2026