RT Journal Article
T1 pkc-1 regulates daf-2 insulin/IGF signalling-dependent control of dauer formation in Caenorhabditis elegans
A1 Monje, José Manuel
A1 Brokate Llanos, Ana María
A1 Pérez-Jiménez, Mercedes M.
A1 Fidalgo, Manuel A.
A1 Muñoz Ruiz, Manuel Jesús
K1 Longevity
K1 Dauer
K1 Insulin
K1 Nuclear hormone
K1 Protein kinase C
K1 C. elegans
AB In Caenorhabditis elegans, the insulin/IGF pathway participates in the decision to initiate dauer development. Dauer is a diapause stage that is triggered by environmental stresses, such as a lack of nutrients. Insulin/IGF receptor mutants arrest constitutively in dauer, an effect that can be suppressed by mutations in other elements of the insulin/IGF pathway or by a reduction in the activity of the nuclear hormone receptor daf-12. We have isolated a pkc-1 mutant that acts as a novel suppressor of the dauer phenotypes caused by insulin/IGF receptor mutations. Interactions between insulin/IGF mutants and the pkc-1 suppressor mutant are similar to those described for daf-12 or the DAF-12 coregulator din-1. Moreover, we show that the expression of the DAF-12 target daf-9, which is normally elevated upon a reduction in insulin/IGF receptor activity, is suppressed in a pkc-1 mutant background, suggesting that pkc-1 could link the daf-12 and insulin/IGF pathways. pkc-1 has been implicated in the regulation of peptide neurosecretion in C. elegans. Although we demonstrate that pkc-1 expression in the nervous system regulates dauer formation, our results suggest that the requirement for pkc-1 in neurosecretion is independent of its role in modulating insulin/IGF signalling. pkc-1 belongs to the novel protein kinase C (nPKC) family, members of which have been implicated in insulin resistance and diabetes in mammals, suggesting a conserved role for pkc-1 in the regulation of the insulin/IGF pathway.
PB Wiley
YR 2011
FD 2011-09-20
LK https://hdl.handle.net/10433/23021
UL https://hdl.handle.net/10433/23021
LA en
NO Monje JM, Brokate-Llanos AM, Pérez-Jiménez MM, Fidalgo MA, Muñoz MJ. pkc-1 regulates daf-2 insulin/IGF signalling-dependent control of dauer formation in Caenorhabditis elegans. Aging Cell. 2011 Dec;10(6):1021-31. doi: 10.1111/j.1474-9726.2011.00747.x. PMID: 21933341.
NO AcknowledgmentsWe dedicate this work to the memory of our colleague and friend Manuel A. Fidalgo. We would like to thank the CGC, Adam Antebi and Thomas Johnson for providing strains and Ikue Mori and Derek S. Sieburth for the pkc-1 neuron promoter plasmids. We would also like to acknowledge Victor Carranco for his excellent technical assistance and John Pearson and Antonio Miranda-Vizuete for their critical reading of this manuscript. This work was supported by the Spanish Ministry of Science (MICINN) BFU2006-07391 ⁄ BMC, theEuropean Regional Development Fund (FEDER) and the Junta de Andalucía Project P07-CVI-02697. JMM was supported by the FPU program of the Spanish Ministry of Science. AMB was supported by a Plan Propio de Investigacio´n fellowship from UPO. MMP was supported by a Junta de Andalucı´a fellowship.
NO Departamento de Biología Molecular e Ingeniería Bioquímica, UPO
NO CABD
DS RIO
RD May 9, 2026