RT Journal Article
T1 Two paralogous EcfG σ factors hierarchically orchestrate the activation of the General Stress Response in Sphingopyxis granuli TFA
A1 Dios Barranco, Rubén de
A1 Rivas Marín, Elena
A1 Santero, Eduardo
A1 Reyes-Ramírez, Francisca
K1 General Stress Response
K1 Extracytoplasmic function (ECF) sigma factors
K1 Transcription
AB Under ever-changing environmental conditions, the General Stress Response (GSR) represents a lifesaver for bacteria in order to withstand hostile situations. In α-proteobacteria, the EcfG-type extracytoplasmic function (ECF) σ factors are the key activators of this response at the transcriptional level. In this work, we address the hierarchical function of the ECF σ factor paralogs EcfG1 and EcfG2 in triggering the GSR in Sphingopyxis granuli TFA and describe the role of EcfG2 as global switch of this response. In addition, we define a GSR regulon for TFA and use in vitro transcription analysis to study the relative contribution of each EcfG paralog to the expression of selected genes. We show that the features of each promoter ultimately dictate this contribution, though EcfG2 always produced more transcripts than EcfG1 regardless of the promoter. These first steps in the characterisation of the GSR in TFA suggest a tight regulation to orchestrate an adequate protective response in order to survive in conditions otherwise lethal.
PB Springer Nature
YR 2020
FD 2020-03-20
LK https://hdl.handle.net/10433/25388
UL https://hdl.handle.net/10433/25388
LA en
NO Scientific Reports
NO This work was supported by grant BIO2014–57545-R, co-funded by the Spanish Ministerio de Educación y Ciencia and the European Regional Development Fund, and by fellowships from the FPU program (Ministerio de Educación y Ciencia, Spain), awarded to Rubén de Dios
NO Universidad Pablo de Olavide. Departamento de Biología Molecular e Ingeniería Bioquímica
DS RIO
RD Apr 23, 2026