RT Journal Article
T1 New methods for the isolation and characterization of biofilm-persistent mutants in Pseudomonas putida
A1 López Sánchez, Aroa
A1 Jiménez-Fernández, Alicia
A1 Calero, Patricia
A1 Gallego, Laura Dolores
A1 Govantes, Fernando
K1 Biofilm development
K1 Pseudomonas
K1 Biofilm dispersal
AB Here we describe two new methods for the genetic characterization of bacterial biofilm development. Firstly, we have designed a microtiter dish-based approach for high-throughput screening of Pseudomonas putida mutants showing increased biofilm under dispersal conditions. Using this method, nine such biofilm-persistent mutants, bearing transposon insertions in four loci: lapG, bifA, mvaB and dksA, were isolated. Secondly, we have developed a serial dilution-based scheme to monitor biofilm development and dispersal in microtiter dish wells in a simple, time-efficient and reproducible manner. Using this method, we showed that (i) mutants in bifA and dksA do not undergo starvation-induced biofilm dispersal in LB or minimal medium, (ii) a mvaB mutant does not disperse the biofilm in LB, but shows a normal dispersal response in minimal medium, and (iii) unlike the lapG mutant, the bifA, mvaB and dksA mutants do not show an increase in biofilm production. The procedures shown here are useful tools for the identification of previously uncharacterized biofilm-related genes and considerably simplify the characterization of biofilm growth phenotypes.
PB John Wiley & Sons
YR 2013
FD 2013-05-01
LK https://hdl.handle.net/10433/25343
UL https://hdl.handle.net/10433/25343
LA en
NO Environmental Microbiology Reports, vol 5, nº5, p. 679-685
NO Universidad Pablo de Olavide. Departamento de Biología Molecular e Ingeniería Bioquímica
DS RIO
RD May 6, 2026