RT Journal Article
T1 Selective Nuclear Pore Complex Removal Drives Nuclear Envelope Division in Fission Yeast
A1 Expósito Serrano, María
A1 Sánchez Molina, Ana
A1 Gallardo Palomo, Paola
A1 Salas-Pino, Silvia
A1 Daga, Rafael
K1 Mitosis
K1 Importin α
K1 Nuclear pore complex
K1 Nuclear envelope
K1 Nuclear envelope division
K1 Central spindle
K1 Midzone membrane domain
K1 ER tubules
K1 Endomembrane sorting
AB An important question in cell biology is how cellular organelles partition during cell division. In organisms undergoingclosed mitosis, the elongation of an intranuclear spindle drives nuclear division, generating twoidentically sized nuclei [1, 2]. However, how the site of nuclear division is determined and the underlyingmechanism driving nuclear envelope (NE) fission remain largely unknown. Here, using the fission yeast, weshow that the microtubule bundler Ase1/PRC1 at the spindle midzone is required for the local concentrationof nuclear pore complexes (NPCs) in the region of the NE in contact with the central spindle. As the spindleelongates during anaphase B, components of these NPCs are sequentially eliminated, and this is accompaniedby the local remodeling of the NE. These two events lead to the eventual removal of NPCs and nucleardivision. In the absence of importin a, NPCs remain stable in this region and no event of NE remodeling isobserved. Consequently, cells fail to undergo nuclear division. Thus, our results highlight a new role of thecentral spindle as a spatial cue that determines the site of nuclear division and point to NPC removal asthe triggering event.
PB Cell Press
YR 2020
FD 2020-08-17
LK https://hdl.handle.net/10433/25241
UL https://hdl.handle.net/10433/25241
LA en
NO Curr Biol. 2020 Aug 17;30(16):3212-3222.e2
NO Proyectos de investigaciónMinisterio de Economía y Competitividad BFU2015-70604-PMinisterio de Economía y Competitividad  PGC2018-099849-B-I00Junta de Andalucía/Consejería de Economía, Conocimiento, Empresas y Universidad/FEDER/UPO UPO-1264663
NO Departamento de Biología Molecular e Ingeniería Bioquímica. Centro Andaluz de Biología del Desarrollo
DS RIO
RD Apr 23, 2026