%0 Journal Article
%A Expósito Serrano, María
%A Sánchez Molina, Ana
%A Gallardo Palomo, Paola
%A Salas-Pino, Silvia
%A Daga, Rafael
%T Selective Nuclear Pore Complex Removal Drives Nuclear Envelope Division in Fission Yeast
%D 2020
%U https://hdl.handle.net/10433/25241
%X An important question in cell biology is how cellular organelles partition during cell division. In organisms undergoingclosed mitosis, the elongation of an intranuclear spindle drives nuclear division, generating twoidentically sized nuclei [1, 2]. However, how the site of nuclear division is determined and the underlyingmechanism driving nuclear envelope (NE) fission remain largely unknown. Here, using the fission yeast, weshow that the microtubule bundler Ase1/PRC1 at the spindle midzone is required for the local concentrationof nuclear pore complexes (NPCs) in the region of the NE in contact with the central spindle. As the spindleelongates during anaphase B, components of these NPCs are sequentially eliminated, and this is accompaniedby the local remodeling of the NE. These two events lead to the eventual removal of NPCs and nucleardivision. In the absence of importin a, NPCs remain stable in this region and no event of NE remodeling isobserved. Consequently, cells fail to undergo nuclear division. Thus, our results highlight a new role of thecentral spindle as a spatial cue that determines the site of nuclear division and point to NPC removal asthe triggering event.
%K Mitosis
%K Importin α
%K Nuclear pore complex
%K Nuclear envelope
%K Nuclear envelope division
%K Central spindle
%K Midzone membrane domain
%K ER tubules
%K Endomembrane sorting
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