RT Journal Article
T1 A subchronic 90-day oral toxicity study of Origanum vulgare essential oil in rats
A1 Llana Ruíz-Cabello, María
A1 Maisanaba, Sara
A1 Puerto, Maria
A1 Pichardo, Silvia
A1 Jos, Angeles
A1 Moyano, Rosario
A1 Cameán, Ana María
K1 Oregano essential oil
K1 Subchronic toxicity
AB Oregano essential oil (Origanum vulgare L. virens) (OEO) is being used in the food industry due to itsuseful properties to develop new active packaging systems. In this concern, the safety assessment of thisnatural extract is of great interest before being commercialized. The European Food Safety Authorityrequests different in vivo assays to ensure the safety of food contact materials. One of these studies is a 90days repeated-dose oral assay in rodents. In the present work, 40 male and 40 female Wistar rats wereorally exposed to 50, 100 and 200 mg/kg body weight (b.w.) OEO during 90 days following the OECDguideline 408. Data revealed no mortality and no treatment-related adverse effects of the OEO in food/water consumption, body weight, haematology, biochemistry, necropsy, organ weight and histopathology.These findings suggest that the oral no-observed-adverse-effect level (NOAEL) of this OEO is200 mg/kg b.w. in Wistar rats, the highest dose tested. In conclusion, the use of this OEO in foodpackaging appears to be safe based on the lack of toxicity during the subchronic study at doses 330-foldhigher than those expected to be in contact consumers in the worst scenario of exposure.
PB Elsevier
YR 2017
FD 2017-01-03
LK https://hdl.handle.net/10433/19834
UL https://hdl.handle.net/10433/19834
LA en
NO A subchronic 90-day oral toxicity study of Origanum vulgare essential oil in rats. M. Llana-Ruiz-Cabello, S. Maisanaba, M. Puerto, S. Pichardo , A. Jos, R. Moyano, A.M. Camean. Food and Chemical Toxicology 101 (2017) 36-47
NO Proyectos de investigaciónFECYT -- EVALUACION DE LA SEGURIDAD PARA CONTACTO ALIMENTARIO DE ENVASES...AGR-7252
NO Area of Toxicology, Faculty of Pharmacy, Universidad de Sevilla
NO Department of Pharmacology, Toxicology and Legal and Forensic Medicine, University of C ordoba
DS RIO
RD May 24, 2026