%0 Journal Article
%A Llana Ruíz-Cabello, María
%A Maisanaba, Sara
%A Puerto, Maria
%A Pichardo, Silvia
%A Jos, Angeles
%A Moyano, Rosario
%A Cameán, Ana María
%T A subchronic 90-day oral toxicity study of Origanum vulgare essential oil in rats
%D 2017
%U https://hdl.handle.net/10433/19834
%X Oregano essential oil (Origanum vulgare L. virens) (OEO) is being used in the food industry due to itsuseful properties to develop new active packaging systems. In this concern, the safety assessment of thisnatural extract is of great interest before being commercialized. The European Food Safety Authorityrequests different in vivo assays to ensure the safety of food contact materials. One of these studies is a 90days repeated-dose oral assay in rodents. In the present work, 40 male and 40 female Wistar rats wereorally exposed to 50, 100 and 200 mg/kg body weight (b.w.) OEO during 90 days following the OECDguideline 408. Data revealed no mortality and no treatment-related adverse effects of the OEO in food/water consumption, body weight, haematology, biochemistry, necropsy, organ weight and histopathology.These findings suggest that the oral no-observed-adverse-effect level (NOAEL) of this OEO is200 mg/kg b.w. in Wistar rats, the highest dose tested. In conclusion, the use of this OEO in foodpackaging appears to be safe based on the lack of toxicity during the subchronic study at doses 330-foldhigher than those expected to be in contact consumers in the worst scenario of exposure.
%K Oregano essential oil
%K Subchronic toxicity
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