RT Journal Article
T1 Crosstalk of Cytokinin with Ethylene and Auxin for Cell Elongation Inhibition and Boron Transport in Arabidopsis Primary Root under Boron Deficiency
A1 Herrera Rodríguez, María Begoña
A1 Camacho-Cristóbal, Juan José
A1 Barrero-Rodríguez, Rafael
A1 Rexach, Jesús
A1 Navarro-Gochicoa, María Teresa
A1 González-Fontes, Agustín
K1 ACS11 gene
K1 AUX1 gene
K1 Boron transporters
K1 Plant hormones
K1 Root elongation
AB Several studies have shown the role of phytohormones in the regulation of root growth of Arabidopsis plants under boron (B) deficiency. Ethylene and auxin play an important role in the control of Arabidopsis primary root cell elongation under short-term B deprivation, whereas cytokinins regulate root growth inhibition under B deficiency by controlling meristem cell proliferation. In this work, we study the possible interaction among cytokinin, ethylene, and auxin in the primary root response to B-deprivation treatment, as well as their possible role in B uptake and transport. Wild type (WT) and two mutants related to auxin and ethylene (aux1 and acs11) Arabidopsis plants were grown in control (10 µM B) or B starvation (0 µM B) treatment, in the absence or presence of trans-zeatin, and their primary root growth was analyzed. The possible interaction between these hormones was also studied by analyzing AUX1 gene expression in the acs11 mutant and ACS11 gene expression in the aux1 mutant. The GUS reporter lines ARR5::GUS, IAA2::GUS, and EBS::GUS were used to observe changes in cytokinin, auxin, and ethylene levels in the root, respectively. The results of this work suggest that cytokinin inhibits root cell elongation under B deficiency through two different mechanisms: (i) an ethylene-dependent mechanism through increased expression of the ACS11 gene, which would lead to increased ethylene in the root, and (ii) an ethylene-independent mechanism through decreased expression of the AUX1 gene, which alters auxin signaling in the meristematic and elongation zones and stele. We also report that changes in the expression of several B transporters occur in response to auxin, ethylene, and cytokinin that may affect the plant B content.
PB MDPI
YR 2022
FD 2022-09-08
LK http://hdl.handle.net/10433/16742
UL http://hdl.handle.net/10433/16742
LA en
NO Plants 2022, 11, 2344. https:// doi.org/10.3390/plants11182344
NO Departamento de Fisiología, Anatomía y Biología Celular, Universidad Pablo de Olavide
DS RIO
RD May 5, 2026