RT Journal Article T1 Valorisation of olea europaea L. Olive Leaves through the evaluation of their extracts: Antioxidant and antimicrobial activity A1 Sánchez-Gutiérrez, Mónica A1 Bascón-Villegas, Isabel A1 Rodríguez, Alejandro A1 Pérez-Rodríguez, Fernando A1 Fernández-Prior, África A1 Rosal Raya, Antonio A1 Carrasco, Elena K1 Agri-food waste K1 Bioactive compounds K1 Polyphenols K1 Foodborne pathogens K1 Microwave assisted extraction K1 Nutraceutical AB Olea europaea L. leaves constitute a source of bioactive compounds with recognized benefits for both human health and technological purposes. In the present work, different extracts from olive leaves were obtained by the application of two extraction methods, Soxhlet and microwave-assisted extraction (MAE), and six solvents (distilled water, ethanolic and glycerol mixtures solvents). MAE was applied under 40, 60 and 80 ◦C for 3, 6.5 and 10 min. The effect of the extraction method, solvent and treatment factors (the latter in MAE) on the total phenol content (TPC), the antioxidant activity (AA) and the phenolic profile of the extracts were all evaluated. The extracts showed high values of TPC (up to 76.1 mg GAE/g DW) and AA (up to 78 mg TE/g DW), with oleuropein being the most predominant compound in all extracts. The Soxhlet extraction method exhibited better yields in TPC than in MAE, although both methods presented comparable AA values. The water MAE extract presented the strongest antimicrobial activity against five foodborne pathogens, with minimum inhibitory concentration (MIC) values ranging from 2.5 to 60 mg/mL. MAE water extract is proposed to be exploited in the food and nutraceutical industry in the frame of a sustainable economy. PB MDPI YR 2021 FD 2021 LK https://hdl.handle.net/10433/22112 UL https://hdl.handle.net/10433/22112 LA en NO Sánchez-Gutiérrez, M.; Bascón-Villegas, I.; Rodríguez, A.; Pérez-Rodríguez, F.; Fernández-Prior, Á.; Rosal, A.; Carrasco, E. Valorisation of Olea europaea L. Olive Leaves through the Evaluation of Their Extracts: Antioxidant and Antimicrobial Activity. Foods 2021, 10, 966. https://doi.org/10.3390/foods10050966 NO The authors are grateful to the Ministry of Economy and Competitiveness of Spain for funding this research through the project CTQ2016-78729-R, to the Andalusian research project PY18-616 (Plan for Research, Development and Innovation 2020), and to the Andalusian knowledge transfer project AT17-5927 co-funded by the European Regional Development Fund instrument (ERDF Andalusian Operative Programme 2014–2020).CTQ2016-78729-RPY18-616AT17-5927 NO Universidad Pablo de Olavide. Departamento de Biología Molecular e Ingeniería Bioquímica DS RIO RD May 10, 2026