RT Journal Article
T1 The Ceramide Synthase Subunit Lac1 Regulates Cell Growth and Size in Fission Yeast
A1 Flor-Parra, Ignacio
A1 Sabido-Bozo, Susana
A1 Ikeda, Atsuko
A1 Hanaoka, Kazuki
A1 Aguilera-Romero, Auxiliadora
A1 Funato, Kouichi
A1 Muñiz. Manuel, 
A1 Lucena, Rafael
K1 Cell growth;
K1 Cell size;
K1 Ceramide synthase;
K1 Lac1;
K1 Lag1;
K1 FIssion yeast
AB Cell division produces two viable cells of a defined size. Thus, all cells require mechanisms to measure growth and trigger cell division when sufficient growth has occurred. Previous data suggest a model in which growth rate and cell size are mechanistically linked by ceramide-dependent signals in budding yeast. However, the conservation of mechanisms that govern growth control is poorly understood. In fission yeast, ceramide synthase is encoded by two genes, Lac1 and Lag1. Here, we characterize them by using a combination of genetics, microscopy, and lipid analysis. We showed that Lac1 and Lag1 co-immunoprecipitate and co-localize at the endoplasmic reticulum. However, each protein generates different species of ceramides and complex sphingolipids. We further discovered that Lac1, but not Lag1, is specifically required for proper control of cell growth and size in Schizosaccharomyces pombe. We propose that specific ceramide and sphingolipid species produced by Lac1 are required for normal control of cell growth and size in fission yeast.
PB MDPI
YR 2021
FD 2021-12-28
LK https://hdl.handle.net/10433/22773
UL https://hdl.handle.net/10433/22773
LA en
NO Int. J. Mol. Sci. 2022, 23(1), 303
NO P18-FRJ-1132
NO VIPPIT-2020-I.5
NO JP19H02922
NO JP21K19088
NO Centro Andaluz de Biología del Desarrollo, Universidad Pablo de Olavide-Consejo Superior de Investigaciones Científica
NO Department of Cell Biology, Faculty of Biology, University of Seville
NO Instituto de Biomedicina de Sevilla (IBiS), Hospital Universitario Virgen del Rocío/CSIC/Universidad de Sevilla
NO School of Applied Biological Science, Graduate School of Integrated Sciences for Life, Hiroshima University
DS RIO
RD May 9, 2026