RT Journal Article
T1 Influence of sampling conditions, salivary flow, and total protein content in uric acid measurements in saliva
A1 González-Hernández, Jorge Miguel
A1 Franco, Lorena
A1 Colomer-Poveda, David
A1 Martínez-Subiela, Silvia
A1 Cugat, Ramón
A1 Cerón, José J.
A1 Márquez, Gonzalo
A1 Martínez Aranda, Luis Manuel
A1 Jiménez-Reyes, Pedro
A1 Tvarijonaviciute, Asta
K1 Squat
K1 Salivettes
K1 Biomarkers
K1 Oxidative stress
AB Uric acid (UA) is the most abundant antioxidant compound in saliva and one of the most sensitive biomarkers for detecting changes in the oxidative status of the organism. The aim of this study was to evaluate the effect of: (i) different methods of saliva sampling and (ii) the correction by salivary flow or total protein on UA concentrations in saliva. Paired saliva (collected by two different methods, passive drooling and using Salivette cotton rolls) and serum samples were obtained from 12 healthy men after the performance of two resistance training exercises of di erent level of effort that can produce different concentrations in UA in saliva. There were no significant differences between values of uric acid in saliva using Salivette and passive drool. Correlations between UA in serum and saliva and increases in UA in saliva after exercise were detected when saliva samples were obtained by passive drool and Salivette and were not corrected by salivary flow or total protein concentration. Therefore for UA measurements in saliva it would not be recommended to normalize the results by salivary flow or protein concentration. This study highlights the importance of choosing an adequate sampling method selection as well as the expression of results when analytes are measured in saliva.
PB MDPI
YR 2019
FD 2019-09-11
LK https://hdl.handle.net/10433/25741
UL https://hdl.handle.net/10433/25741
LA en
NO Antioxidants, vol 8, p. 389
NO Esta investigación fue financiada por la Fundación Séneca de la Región de Murcia, subvención número 19894/GERM/15.
NO Departamento de Deporte e Informática
NO Universidad Católica de Murcia (UCAM), España
DS RIO
RD May 9, 2026