RT Journal Article
T1 Effects of the microtubule nucleator Mto1 on chromosomal movement, DNA repair, and sister chromatid cohesion in fission yeast
A1 Zhurinsky, Jacob
A1 Salas-Pino, Silvia
A1 Iglesias Romero, Ana Belén
A1 Torres-Méndez, Antonio
A1 Knapp, Benjamin
A1 Flor-Parra, Ignacio
A1 Wang, Jiyong
A1 Bao, Kehan
A1 Jia, Songtao
A1 Chang, Fred
A1 Daga, Rafael
K1 DNA repair
K1 Mto1
K1 Fission yeast
K1 Microtubules
AB Although the function of microtubules (MTs) in chromosomal segregation during mitosis is well characterized, much less is known about the role of MTs in chromosomal functions during interphase. In the fission yeast Schizosaccharomyces pombe, dynamic cytoplasmic MT bundles move chromosomes in an oscillatory manner during interphase via linkages through the nuclear envelope (NE) at the spindle pole body (SPB) and other sites. Mto1 is a cytoplasmic factor that mediates the nucleation and attachment of cytoplasmic MTs to the nucleus. Here, we test the function of these cytoplasmic MTs and Mto1 on DNA repair and recombination during interphase. We find that mto1Δ cells exhibit defects in DNA repair and homologous recombination (HR) and abnormal DNA repair factory dynamics. In these cells, sister chromatids are not properly paired, and binding of Rad21 cohesin subunit along chromosomal arms is reduced. Our findings suggest a model in which cytoplasmic MTs and Mto1 facilitate efficient DNA repair and HR by promoting dynamic chromosomal organization and cohesion in the nucleus.
PB ASCB
YR 2019
FD 2019-09-30
LK https://hdl.handle.net/10433/22769
UL https://hdl.handle.net/10433/22769
LA en
NO Zhurinsky, J., Salas-Pino, S., Iglesias-Romero, A. B., Torres-Mendez, A., Knapp, B., Flor-Parra, I., Wang, J., Bao, K., Jia, S., Chang, F., Daga, R. R., & Bloom, K. S. (2019). Effects of the microtubule nucleator Mto1 on chromosomal movement, DNA repair, and sister chromatid cohesion in fission yeast. Molecular Biology of the Cell, 30(21), 2695–2708.
NO Centro Andaluz de Biologia del Desarrollo, Universidad Pablo de OlavideM
NO Department of Microbiology and Immunology, Columbia University College of Physicians and SurgeonsM
NO Department of Cell and Tissue Biology, University of California, San FranciscoM
NO Department of Biological Science, Columbia University Medical CenterM
DS RIO
RD May 9, 2026