RT Journal Article
T1 Isotopic labelling-based analysis elucidates biosynthesis pathways in Saccharomyces cerevisiae for Melatonin, Serotonin and Hydroxytyrosol formation
A1 Gallardo-Fernández, M.
A1 Valls-Fonayet, J.
A1 Valero Blanco, Eva María
A1 Hornedo-Ortega, R.
A1 Richard, T.
A1 Troncoso, A. M.
A1 Garcia-Parrilla, M. C.
K1 Yeast
K1 Nitrogen
K1 Indolic compounds
K1 Polyphenol
K1 Alcoholic fermentation
K1 HRMS
AB Yeasts can synthetise bioactive compounds such as Melatonin (MEL), Serotonin (SER) and Hydroxytyrosol (HT). Deciphering the mechanisms involved in their formation can lead to exploit this fact to increase the bioactive potential of fermented beverages. Quantitative analysis using labelled compounds, 15-N2 L-tryptophan and 13-C tyrosine, allowed tracking the formation of the above-mentioned bioactive compounds during the alcoholic fermentation of synthetic must by two different Saccharomyces cerevisiae strains. Labelled and unlabelled MEL, SER and HT were undoubtedly identified and quantified by High Resolution Mass Spectrometry (HRMS). Our results prove that there are at least two pathways involved in MEL  biosynthesis by yeast. One starts with tryptophan as precursor being known for the vertebrates’ pathway. Additionally, MEL is produced from SER which in turn is consistent with the plants’ biosynthesis pathway. Concerning HT, it can be formed both from labelled tyrosine and from intermediates of the Erlich pathway.
PB Elsevier
YR 2021
FD 2021-12-07
LK https://hdl.handle.net/10433/25334
UL https://hdl.handle.net/10433/25334
LA en
NO Food Chemistry 374 (2022) 131742
NO Universidad Pablo de Olavide. Departamento de Biología Molecular e Ingeniería Bioquímica
DS RIO
RD May 9, 2026