RT Journal Article
T1 Microtubule polymerase and processive plus-end tracking functions originate from distinct features within TOG domain arrays
A1 Cook, Brian D.
A1 Chang, Fred
A1 Flor-Parra, Ignacio
A1 Al-Bassam, Jawdat
K1 Microtubule
K1 XMAP215
K1 Fission yeast
K1 Polymerase
AB XMAP215/Stu2/Alp14 accelerates tubulin polymerization while processively tracking microtubule (MT) plus ends via tumor overexpressed gene (TOG) domain arrays. It remains poorly understood how these functions arise from tubulin recruitment, mediated by the distinct TOG1 and TOG2 domains, or the assembly of these arrays into large square complexes. Here, we describe a relationship between MT plus-end tracking and polymerase functions revealing their distinct origin within TOG arrays. We study Alp14 mutants designed based on structural models, with defects in either tubulin recruitment or self-organization. Using in vivo live imaging in fission yeast and in vitro MT dynamics assays, we show that tubulins recruited by TOG1 and TOG2 serve concerted, yet distinct, roles in MT plus-end tracking and polymerase functions. TOG1 is critical for processive plus-end tracking, whereas TOG2 is critical for accelerating tubulin polymerization. Inactivating interfaces that stabilize square complexes lead to defects in both processive MT plus-end tracking and polymerase. Our studies suggest that a dynamic cycle between square and unfurled TOG array states gives rise to processive polymerase activity at MT plus ends.
PB ASCB
YR 2019
FD 2019-05-30
LK https://hdl.handle.net/10433/22770
UL https://hdl.handle.net/10433/22770
LA en
NO Cook, B. D., Chang, F., Flor-Parra, I., Al-Bassam, J., & Surrey, T. (2019). Microtubule polymerase and processive plus-end tracking functions originate from distinct features within TOG domain arrays. Molecular Biology of the Cell, 30(12), 1490–1504.
NO NIH-T32-GM00737
NO NIH-GM110283
NO NSF-1615991
NO NIH-GM115185
NO Centro Andaluz de Biología del Desarrollo, Universidad Pablo de Olavide/CSIC/Junta de Andalucía, 41013 Seville, Spain
NO Department of Molecular Cellular Biology, University of California, Davis, Davis, CA 95616
NO Department of Cell and Tissue Biology, University of California, San Francisco, San Francisco, CA 94143
DS RIO
RD May 10, 2026