RT Journal Article
T1 Dynamic changes in the cerebellar-interpositus/red-nucleus-motoneuron pathway during motor learning
A1 Sánchez-Campusano, Raudel
A1 Gruart, Agnès
A1 Delgado-García, J.M.
K1 Cat
K1 Mice
K1 Classical eyeblink conditioning
K1 Facial motoneuron
K1 Red nucleus
K1 Cerebellar interpositus nucleus
K1 Meta-analysis
AB Understanding the role played by the cerebellum in the genesis and control of learned motor responses requires a precise knowledge of interdependent relationships between kinetic neural commands and the performance (kinematics) of the acquired movements. The eyelid motor system is a useful model for studying how simple motor responses are generated and performed. Here, we recorded the activity of interpositus, red nucleus, and/or facial motor neurons during classical eyeblink conditioning, using a delay paradigm. Experiments were carried out in behaving cats, and in conscious wild-type and (Purkinje cell devoid) Lurcher mice. Kinetic variables were determined by recording the firing activities of identified neurons at the mentioned nuclei, whilst kinematic variables were selected from the electromyographic activity of the orbicularis oculi muscle and/or from eyelid position recorded during the conditioned-stimulus/ unconditioned-stimulus interval. Whereas motoneurons encoded eyelid kinematics for acquired eyelid responses, interpositus, and red nucleus neurons did not directly encode eyelid performance, and the dynamic association between their neuronal activities was barely significant (from moderate to weak correlation, nonlinear coupling with high asymmetry, and neural firing activities that always lagged the beginning of the conditioned response). Nevertheless, interpositus and red nucleus neurons seem to play a modulating role in the dynamic control of this type of learned motor response, and present interesting adaptive properties in Lurcher mice. The analytical procedures proposed here could be very helpful in defining the functional state corresponding to each stage across the acquisition of new motor and cognitive abilities.
PB Springer Science+Business Media, LLC 2010
YR 2011
FD 2011-12
LK https://hdl.handle.net/10433/19865
UL https://hdl.handle.net/10433/19865
LA en
NO Cerebellum, vol 10, nº 4, p. 702-710
NO En este artículo se avanza en el estudio de la red cerebelo/núcleo-interpósito/núcleo-rojo/motoneurona desde un enfoque de meta-análisis ya que se integraron los resultados de estudios realizados en gatos y ratones para poder establecer comparaciones funcionales y llegar a conclusiones más generalizadas sobre los mecanismos que subyacen a la generación de las respuestas motoras aprendidas. Pero no sólo eso, sino que también se emplearon ratones Lurcher sin células de Purkinje del cerebelo para compararlos con ratones controles. Los resultados sugieren que las neuronas del núcleo interpósito del cerebelo y las del núcleo rojo desempeñan un papel modulador con interesantes propiedades adaptativas en ratones Lurcher. Los procedimientos analíticos propuestos aquí podrían ser de gran ayuda para definir el estado funcional correspondiente a cada etapa durante la adquisición de nuevas habilidades motoras y cognitivas.
NO MICINN-BFU2007-67173MICINN-BFU2008-00899BIO122/CVI2487
NO Departamento de Fisiología, Anatomía y Biología Celular
DS RIO
RD May 4, 2026