RT Journal Article
T1 Molybdenum cofactor (chlorate-resistant) mutants of Klebsiella pneumoniae M5al can use hypoxanthine as the sole nitrogen source
A1 Casadesus, Josep
A1 Stewart, Valley
A1 Garzón, Andrés
A1 Flores, Amando
K1 Klebsiella
K1 Molibdenum Cofactor
K1 Nitrogen Assimilation
K1 Chlorate-resistant
AB Selection for chlorate resistance yields mol (formerly chl) mutants with defects in molybdenum cofactor synthesis. Complementation and genetic mapping analyses indicated that the Klebsiella pneumoniae mol genes are functionally homologous to those of Escherichia coli and occupy analogous genetic map positions. Hypoxanthine utilization in other organisms requires molybdenum cofactor as a component of xanthine dehydrogenase, and thus most chlorate-resistant mutants cannot use hypoxanthine as a sole source of nitrogen. Surprisingly, the K. pneumoniae mol mutants and the mol+ parent grew equally well with hypoxanthine as the sole nitrogen source, suggesting that K. pneumoniae has a molybdenum cofactor-independent pathway for hypoxanthine utilization.
PB American Society for Microbiology
SN 0021-9193
YR 1992
FD 1992-10
LK http://hdl.handle.net/10433/731
UL http://hdl.handle.net/10433/731
LA en
NO Journal of Bacteriology  vol. 174 nº 19, p. 6298-6302
NO Departamento de Genética, Universidad de Sevilla, Spain.
DS RIO
RD May 7, 2026