RT Journal Article
T1 β-Naphthoflavone and ethanol reverse mitochondrial dysfunction in a parkinsonian model of neurodegeneration
A1 Fernández Abascal, Jesús
A1 Chiaino, Elda
A1 Frosini, Maria
A1 Davey, Gavin P
A1 Valoti, Massimo
K1 CYP 2D6
K1 CYP 2E1
K1 CYP induction
K1 Cytochrome P-450 system
K1 MPP+ toxicity
K1 Mitochondrial kinetics
K1 Neurodegeneration
K1 Neuroprotection
AB The 1-methyl-4-phenylpyridinium (MPP+) is a parkinsonian-inducing toxin that promotes neurodegeneration of dopaminergic cells by directly targeting complex I of mitochondria. Recently, it was reported that some Cytochrome P450 (CYP) isoforms, such as CYP 2D6 or 2E1, may be involved in the development of this neurodegenerative disease. In order to study a possible role for CYP induction in neurorepair, we designed an in vitro model where undifferentiated neuroblastoma SH-SY5Y cells were treated with the CYP inducers β-naphthoflavone (βNF) and ethanol (EtOH) before and during exposure to the parkinsonian neurotoxin, MPP+. The toxic effect of MPP+ in cell viability was rescued with both βNF and EtOH treatments. We also report that this was due to a decrease in reactive oxygen species (ROS) production, restoration of mitochondrial fusion kinetics, and mitochondrial membrane potential. These treatments also protected complex I activity against the inhibitory effects caused by MPP+, suggesting a possible neuroprotective role for CYP inducers. These results bring new insights into the possible role of CYP isoenzymes in xenobiotic clearance and central nervous system homeostasis.
PB Multidisciplinary Digital Publishing Institute (MDPI)
YR 2020
FD 2020-05-31
LK https://hdl.handle.net/10433/22761
UL https://hdl.handle.net/10433/22761
LA en
NO Fernandez-Abascal, J., Chiaino, E., Frosini, M., Davey, G. P., & Valoti, M. (2020). β-Naphthoflavone and Ethanol Reverse Mitochondrial Dysfunction in A Parkinsonian Model of Neurodegeneration. International Journal of Molecular Sciences, 21(11), 3955. https://doi.org/10.3390/ijms21113955
NO Universidad de Siena
DS RIO
RD May 9, 2026