RT Journal Article T1 Legume living mulch for afforestation in agricultural land in Southern Spain A1 Navarro Cerrillo, Rafael M A1 Ariza, David A1 González, Luis A1 del Campo, Antonio A1 Arjona, Miguel A1 Ceacero Ruiz, Carlos Juan K1 Afforestation K1 Living mulch K1 Medicago rugosa K1 Mediterranean species K1 Weed control AB Weed control is essential for a successful establishment and growth of tree seedlings in former agricultural land. Weed control methods are effective but can be costly in terms of time, damage to non-target vegetation, or increased soil erosion. Alternatively, some living mulches can exclude undesirable vegetation, protect the soil, compete minimally with associated trees, and supplement soil nitrogen, but there is a lack of knowledge on living mulch systems in Mediterranean afforestation. Thus, the objective of the present study was to evaluate the effects on Holm oak (Quercus ilex L.), mastic tree (Pistacia lentiscus L.), wild olive (Olea europaea L. var. sylvestris Brot.) and terebinth (Pistacia terebinthus L.) seedlings of wrinkled medick (Medicago rugosa Desr.) mulch. Survival, growth, photosynthesis, foliar nutrient and soil parameters were measured during the first year. 36 months after planting, seedlings in the living mulch had survival rates of between 60% for mastic tree and 8.3% for Holm oak, compared with survival rates of 70% in the mechanical treatment for mastic tree and 2% for Holm oak. Photosynthesis and foliar nutrient concentrations were improved by the living mulch treatment. The soil under the living mulch had higher CEC, soil organic matter levels and nitrogen content in comparison to the cultivated soil. The response of living mulch differs between species and environmental conditions but our study suggests a positive effect due to soil protection. Living mulch may be a promise alternative for use in Mediterranean afforestation programs. PB Elsevier YR 2008 FD 2008 LK https://hdl.handle.net/10433/19589 UL https://hdl.handle.net/10433/19589 LA en NO Agricultural, Fisheries and Food Department of Junta de Andalusia NO Departamento de Fisiología, Anatomía y Biología Celular, Universidad Pablo de Olavide, E-41013, Sevilla, Spain DS RIO RD May 9, 2026