RT Journal Article
T1 Acute Heat Stress Leads to Reversible Aggregation of Nuclear Proteins into Nucleolar Rings in Fission Yeast
A1 Gallardo Palomo, Paola
A1 Real-Caldero, Paula
A1 Flor-Parra, Ignacio
A1 Salas-Pino, Silvia
A1 Daga, Rafael
K1 Heat stress response
K1 Hsf1
K1 Hsp104
K1 Heat shock
K1 Protein aggregation
K1 Nucleolus
K1 Nuclear pore complex
K1 mRNA
K1 Cell growth;
K1 Cell cycle
AB Upon acute heat stress (HS), overall mRNA transcription, processing, and export are inhibited, leading to cell growth arrest. However, how cells turn off mRNA metabolism is not fully understood. Here, we show that acute HS results in the segregation and aggregation of multiple nuclear and nucleolar proteins into ring-like structures located at the nucleolar periphery (nucleolar rings [NuRs]). NuRs sequester essential factors required for nuclear mRNA metabolism and nuclear pore complex function, as well as cell-cycle regulators. When cells are switched back to growing temperatures, NuRs disaggregate, and their components relocate to their functional environments in an Hsf1- and Hsp104-dependent manner, and concomitantly with the reinitiation of cell growth. These findings highlight the contribution of reversible protein aggregation to the inhibition of overall RNA-related activities in the nucleus and its functional relevance in the maintenance of cellular homeostasis during acute HS.
PB Cell Press
YR 2020
FD 2020-11-10
LK https://hdl.handle.net/10433/22772
UL https://hdl.handle.net/10433/22772
LA en
NO Gallardo, P., Real-Calderón, P., Flor-Parra, I., Salas-Pino, S., & Daga, R. R. (2020). Acute Heat Stress Leads to Reversible Aggregation of Nuclear Proteins into Nucleolar Rings in Fission Yeast. Cell Reports (Cambridge), 33(6)Cell Reports Volume 33, Issue 6, 10 November 2020,
NO FECYT -- DINAMICA DE LA ARQUITECTURA NUCLEAR DURANTE EL CICLO CELULAR EN...
NO PGC2018- 099849-B-I00
NO UPO-1264663
NO Centro Andaluz de Biología del Desarrollo, Universidad Pablo de Olavide-Consejo Superior de Investigaciones Científicas-Junta de Andalucía
DS RIO
RD May 9, 2026