RT Journal Article
T1 Boron Deficiency Increases Cytosolic Ca2+ Levels Mainly via Ca2+ Influx from the Apoplast in Arabidopsis thaliana Roots
A1 Quiles-Pando, Carlos
A1 Navarro Gochicoa, María Teresa
A1 Herrera Rodríguez, María Begoña
A1 Camacho-Cristóbal, Juan José
A1 González-Fontes, Agustín
A1 Rexach, Jesús
K1 Apoplastic calcium
K1 Boron deficiency
K1 Calcium signaling
K1 Cytosolic calcium
K1 Cameleon YC3.6
K1 Arabidopsis thaliana
AB Abstract: Boron (B) is a micronutrient for plant development, and its deficiency alters many physiological processes. However, the current knowledge on how plants are able to sense the B-starvation signal is still very limited. Recently, it has been reported that B deprivation induces an increase in cytosolic calcium concentration ([Ca2+]cyt) in Arabidopsis thaliana roots. The aim of this work was to research in Arabidopsis whether [Ca2+]cyt is restored to initial levels when B is resupplied and elucidate whether apoplastic Ca2+ is the major source for B-deficiency-induced rise in [Ca2+]cyt.The use of chemical compounds a ecting Ca2+ homeostasis showed that the rise in root [Ca2+]cyt induced by B deficiency was predominantly owed to Ca2+ influx from the apoplast through plasma membrane Ca2+ channels in an IP3-independent manner. Furthermore, B resupply restored the root [Ca2+]cyt. Interestingly, expression levels of genes encoding Ca2+ transporters (ACA10, plasma membrane PIIB-type Ca2+-ATPase; and CAX3, vacuolar cation/proton exchanger) were upregulated by ethylene glycol tetraacetic acid (EGTA) and abscisic acid (ABA). The results pointed out that ACA10, and especially CAX3, would play a major role in the restoration of Ca2+ homeostasis after 24 h of B deficiency.
PB MDPI
YR 2019
FD 2019-05-09
LK https://hdl.handle.net/10433/22146
UL https://hdl.handle.net/10433/22146
LA en
NO This work was supported by the Ministerio de Ciencia e Innovación (BFU2012-37445 and BFU2017-88811-P) and Junta de Andalucía (BIO-266 and P09-CVI-4721), Spain.
NO Departamento de Fisiología, Anatomía y Biología Celular. Universidad Pablo de Olavide.
DS RIO
RD Apr 24, 2026