RT Journal Article
T1 New Method for Simultaneous Determination of Microcystins and Cylindrospermopsin in Vegetable Matrices by SPE-UPLC-MS/MS
A1 Diez-Quijada, Leticia
A1 Guzmán-Guillén, Remedios
A1 Prieto, Ana Isabel
A1 Llana Ruíz-Cabello, María
A1 Campos, Alexandre
A1 Vasconcelos, Vitor
A1 Jos, Angeles
A1 Cameán, Ana María
K1 Microcystins;
K1 Cylindrospermopsin;
K1 UPLC-MS/MS
AB Cyanotoxins are a large group of noxious metabolites with different chemical structureand mechanisms of action, with a worldwide distribution, producing effects in animals, humans,and crop plants. When cyanotoxin-contaminated waters are used for the irrigation of ediblevegetables, humans can be in contact with these toxins through the food chain. In this work,a method for the simultaneous detection of Microcystin-LR (MC-LR), Microcystin-RR (MC-RR),Microcystin-YR (MC-YR), and Cylindrospermopsin (CYN) in lettuce has been optimized andvalidated, using a dual solid phase extraction (SPE) system for toxin extraction and ultra-performanceliquid chromatography-tandem mass spectrometry (UPLC-MS/MS) for analysis. Results showedlinear ranges (5–50 ng g􀀀1 f.w.), low values for limit of detection (LOD) (0.06–0.42 ng g􀀀1 f.w.),and limit of quantification (LOQ) (0.16–0.91 ng g􀀀1 f.w.), acceptable recoveries (41–93%), and %RSDIPvalues for the four toxins. The method proved to be robust for the three variables tested. Finally,it was successfully applied to detect these cyanotoxins in edible vegetables exposed to cyanobacterialextracts under laboratory conditions, and it could be useful for monitoring these toxins in ediblevegetables for better exposure estimation in terms of risk assessment.
PB MDPI
YR 2018
FD 2018-10-08
LK https://hdl.handle.net/10433/19837
UL https://hdl.handle.net/10433/19837
LA en
NO 5.	New method for simultaneous determination of microcystins and Cylindrospermopsin in vegetable matrices by SPE-UPLC-MS/MS. Díez-Quijada, L., Guzmán-Guillén, R., Prieto Ortega, A.I., Llana-Ruiz-Cabello, M., Campos, A., Jos, Á., Cameán, A.M. Toxins, 2018, 10(10), 406.
NO Proyectos de investigaciónAGL2015-64558-R, MINECO/FEDER,FCT project UID/Multi/04423/2013(SFRH/BPD/103683/2014)
NO Area of Toxicology, Faculty of Pharmacy, University of Sevilla,
NO CIIMAR/CIMAR—Interdisciplinary Centre of Marine and Environmental Research, University of Porto
NO Faculty of Sciences, University of Porto,
DS RIO
RD May 25, 2026