RT Journal Article
T1 Ustilago maydis Secreted Endo-Xylanases Are Involved in Fungal Filamentation and Proliferation on and Inside Plants
A1 Moreno-Sánchez, Ismael
A1 Pejenaute Ochoa, María Dolores
A1 Navarrete Ruiz de Clavijo, Blanca
A1 Ramos Barrales, Ramón
A1 Ibeas, José
K1 PCWDE
K1 Smut fungi
K1 Pathogenesis
AB Plant pathogenic fungi must be able to degrade host cell walls in order to penetrate and invade plant tissues. Among the plant cell wall degrading enzymes (PCWDEs) produced, xylanases are of special interest since its degradation target, xylan, is one of the main structural polysaccharides in plant cell walls. In the biotrophic fungus Ustilago maydis, attempts to characterize PCWDEs required for virulence have been unsuccessful, most likely due to functional redundancy. In previous high-throughput screening, we found one xylanase to be important for U. maydis infection. Here, we characterize the entire U. maydis endo-xylanase family, comprising two enzymes from the glycoside hydrolase (GH) 10 family, Xyn1 and Xyn2, one from GH11, Xyn11A, and one from GH43, Xyn3. We show that all endo-xylanases except Xyn3 are secreted and involved in infection in a non-redundant manner, suggesting different roles for each xylanase in this process. Taking a closer look inside the plant during the pathogenic process, we observed that all secreted xylanases were necessary for fungal proliferation. Finally, we found that at least Xyn11A accumulated in the apoplast of the infected plant after three days, highlighting the role of these enzymes as important secreted proteins during fungal proliferation inside plant tissues.
PB MDPI
YR 2021
FD 2021-12-15
LK https://hdl.handle.net/10433/26117
UL https://hdl.handle.net/10433/26117
LA en
NO Journal of Fungi, 7(12), 1081
NO Centro Andaluz de Biología del Desarrollo (CABD), Universidad Pablo de Olavide-CSIC-Junta de Andalucía, Ctra. Utrera km.1, 41013 Seville, Spain
DS RIO
RD May 7, 2026