RT Journal Article
T1 Boron Deficiency Increases Cytosolic Ca2+ Levels Mainly via Ca2+ Influx from the Apoplast in Arabidopsis thaliana Roots
A1 Quiles-Pando, Carlos
A1 Navarro-Gochicoa, M. Teresa
A1 Herrera Rodríguez, María Begoña
A1 Camacho-Cristóbal, Juan José
A1 González-Fontes, Agustín
A1 Rexach, Jesús
K1 Apoplastic calcium
K1 Boron deficiency
K1 Calcium signaling
K1 Cytosolic calcium
K1 Cameleon YC3.6
K1 Arabidopsis thaliana
AB Boron (B) is a micronutrient for plant development, and its deficiency alters manyphysiological processes. However, the current knowledge on how plants are able to sense theB-starvation signal is still very limited. Recently, it has been reported that B deprivation induces anincrease in cytosolic calcium concentration ([Ca2+]cyt) in Arabidopsis thaliana roots. The aim of thiswork was to research in Arabidopsis whether [Ca2+]cyt is restored to initial levels when B is resuppliedand elucidate whether apoplastic Ca2+ is the major source for B-deficiency-induced rise in [Ca2+]cyt.The use of chemical compounds affecting Ca2+ homeostasis showed that the rise in root [Ca2+]cytinduced by B deficiency was predominantly owed to Ca2+ influx from the apoplast through plasmamembrane Ca2+ channels in an IP3-independent manner. Furthermore, B resupply restored theroot [Ca2+]cyt. Interestingly, expression levels of genes encoding Ca2+ transporters (ACA10, plasmamembrane PIIB-type Ca2+-ATPase; and CAX3, vacuolar cation/proton exchanger) were upregulatedby ethylene glycol tetraacetic acid (EGTA) and abscisic acid (ABA). The results pointed out thatACA10, and especially CAX3, would play a major role in the restoration of Ca2+ homeostasis after24 h of B deficiency.
PB MDPI
YR 2019
FD 2019
LK https://hdl.handle.net/10433/19612
UL https://hdl.handle.net/10433/19612
LA en
NO Quiles-Pando, C., Navarro-Gochicoa, M. T., Herrera-Rodríguez, M. B., Camacho-Cristóbal, J. J., González-Fontes, A., & Rexach, J. (2019). Boron Deficiency Increases Cytosolic Ca2+ Levels Mainly via Ca2+ Influx from the Apoplast in Arabidopsis thaliana Roots. International Journal of Molecular Sciences, 20(9), 2297. https://doi.org/10.3390/ijms20092297
NO Ministerio de Ciencia e Innovación (BFU2012-37445 y BFU2017-88811-P). Junta de Andalucía (BIO-266 y P09-CVI-4721)
NO Departamento de Fisiología, Anatomía y Biología Celular
DS RIO
RD Apr 25, 2026