RT Journal Article
T1 In vitro toxicity evaluation of new silane-modified clays and the migration extract from a derived polymer-clay nanocomposite intended to food packaging applications
A1 Maisanaba, Sara
A1 Guzmán-Guillén, Remedios
A1 Puerto, María
A1 Gutiérrez-Praena, Daniel
A1 Ortuño, Natalia
A1 Jos, Ángeles
K1 Silane modified-clay
K1 Montmorillonite
K1 Nanocomposite
K1 Caco-2 cells
K1 Toxicity
AB The clay montmorillonite (Mt) is among the nanofillers more frequently used for food packaging applications.The organomodification of clays with different modifiers, such as silanes, is an important step inthe preparation of improved polymer/clay materials known as nanocomposites. However, the toxicologicaldata about these nanofillers is still scarce. In the present study, an in vitro toxicological evaluation inCaco-2 cells of two silane-modified clays based on Mt, Clay3 and Clay4 (0–250  g/ml), was performed.The cytotoxicity, cell death, genotoxicity and oxidative stress produced by both organoclays were evaluatedafter 24 and 48 h of exposure. Moreover, the migration extracts obtained from nanocomposites ofpolypropylene (PP) + Clay3 and only PP were also investigated. Only Clay4 induced cytotoxicity, showinga reduction of cell viability to 63% of the control, as well as oxidative stress in a concentration-dependentmanner. Regarding the PP-Clay3 migration extract, no cytotoxic effects were observed after exposureto the tested concentrations (0–100%). Moreover, significant differences in the presence of Ca, Mg andSi compared to the PP extract were obtained, although migration levels were in accordance with thefood contact materials regulation. These findings indicate that a case-by-case toxicological assessmentof organoclays should be performed.
PB Elsevier
YR 2017
FD 2017-08-02
LK https://hdl.handle.net/10433/19706
UL https://hdl.handle.net/10433/19706
LA en
NO Journal of Hazardous Materials 341 (2018) 313–320
NO Proyectos de investigaciónJunta de Andalucía (AGR5969)
NO Area of Toxicology, Faculty of Pharmacy, University of Sevilla
NO Area of Packaging Materials and Systems, ITENE
DS RIO
RD Apr 23, 2026