RT Journal Article T1 Commercial wine yeast nitrogen requirement influences the production of secondary metabolites (aroma, hydroxytyrosol, melatonin and other bioactives) during alcoholic fermentation A1 González-Ramírez. Marina, A1 Kazakova, Julia A1 García-Serrano, Pedro A1 Ubeda, Cristina A1 Valero Blanco, Eva María A1 Cerezo, Ana B. A1 Troncoso, Ana M. A1 García-Parrilla, M. Carmen K1 YAN K1 Hydroxytyrosol K1 Melatonin K1 Volatile compounds K1 Mass spectrometry AB During alcoholic fermentation, Saccharomyces cerevisiae synthesizes different compounds, which are crucial for product quality: volatile compounds with sensory impact, and bioactive compounds such as melatonin (MEL) and hydroxytyrosol (HT), linked to health benefits. As many of these compounds are related with yeast’s nitrogen metabolism, their production have been studied in four different commercial strains with different nitrogen requirement (Red Fruit, Uvaferm VRB, Lalvin Rhone 2323 and Lalvin QA23) being, Uvaferm UVR the higher nitrogen demander strain. All strains produced the secondary metabolites, notably Uvaferm UVR produced the highest HT concentration, despite its low growth. Uvaferm UVR emerged also as a significant producer of MEL, indicating a potential role in fermentation related stress. Moreover, Uvaferm UVR shows the highest total concentrations of volatile compounds. Multivariate analysis revealed distinct clustering based on nitrogen requirements of the strains, highlighting the strain-dependent metabolic responses. PB Elsevier YR 2024 FD 2024-06-20 LK https://hdl.handle.net/10433/25353 UL https://hdl.handle.net/10433/25353 LA en NO International Journal of Food Microbiology 421 (2024) 110788 NO Universidad Pablo de Olavide. Departamento de Biología Molecular e Ingeniería Bioquímica DS RIO RD May 9, 2026