RT Journal Article
T1 Absorption, metabolism, and excretion of fermented orange juice (poly)phenols in rats
A1 Escudero López, Blanca
A1 Calani, Luca
A1 Fernández-Pachón, María-Soledad
A1 Ortega de la Torre, María de los Ángeles
A1 Brighenti, Furio
A1 Crozier, Alan
A1 Del Rio, Daniele
K1 Orange juice
K1 Flavanones
K1 Hydroxycinnamates
K1 Bioavailability
K1 Rats
K1 Colon
K1 Microbiota
K1 Urinary excretion
AB Two milliliters of a fermented, pasteurized orange juice containing 1% alcohol and 2.3 lmol of (poly)phenolic compounds was fed to rats by gavage after which plasma and urine collected over a 36 h period were analyzed by UHPLCmass spectrometry. The main constituents in the juice were hesperetin and naringenin-O-glycosides, apigenin-6,8-C-diglucoside, and ferulic acid-40-O-glucoside. Plasma contained seven flavanone glucuronides, with the principal metabolites, naringenin-7-O-glucuronide, naringenin-40 -O-glucuronide, and an isosakuranetin-O-glucuronide, peaking 6 h after intake at concentrations of 10 nmol/L. Urinary excretion of four hesperetin glucuronides was equivalent to 0.28% of intake while that of the two naringenin glucuronides was 2.8% of intake.The plasma and urine data suggest that while some absorption occurred in the small intestine, the main site of uptake was the colon. Urine also contained dihydroferulic acid-4’-Oglucuronide and dihydroferulic acid-40-O-sulfate which were excreted in quantities corresponding to 48.2% of the ingested ferulic acid-40-glucoside. This indicates that the hydroxycinnamate is much more bioavailable than the flavanones in the rat model. Conversion of the ferulic acid glucoside to the dihydroferulic acid metabolites involves the action of colonic microbial glycosidases and reductases/hydrogenases followed by postabsorption phase II metabolism before renal excretion.
PB John Wiley & Sons
YR 2013
FD 2013-11-20
LK https://hdl.handle.net/10433/19608
UL https://hdl.handle.net/10433/19608
LA en
NO Escudero-López, B., Calani, L., Fernández-Pachón, M.-S., Ortega, Á., Brighenti, F., Crozier, A. and Del Rio, D. (2014), Absorption, metabolism, and excretion of fermented orange juice (poly)phenols in rats. BioFactors, 40: 327-335. https://doi.org/10.1002/biof.1152
NO FECYT -- Elaboración De Una Novedosa Bebida De Naranja De Baja Graduació...
NO Área de Nutrición y Bromatología. Departamento de Biología Molecular e Ingeniería Bioquímica. Universidad Pablo de Olavide
DS RIO
RD May 4, 2026