RT Journal Article
T1 Nuclear Mechanics in the Fission Yeast
A1 Gallardo Palomo, Paola
A1 Ramos Barrales, Ramón
A1 Daga, Rafael
A1 Salas-Pino, Silvia
K1 Nucleus
K1 Nuclear mechanics
K1 Nuclear envelope
K1 Nuclear architecture
K1 Microtubule (MT) cytoskeleton
K1 Linker of nucleoskeleton and cytoskeleton (LINC) complex
K1 Inner nuclear membrane (INM) proteins
K1 Genome 3D organization
K1 Chromatin domains
K1 MT pushing forces; chromatin
AB In eukaryotic cells, the organization of the genome within the nucleus requires the nuclear envelope (NE) and its associated proteins. The nucleus is subjected to mechanical forces produced by the cytoskeleton. The physical properties of the NE and the linkage of chromatin in compacted conformation at sites of cytoskeleton contacts seem to be key for withstanding nuclear mechanical stress. Mechanical perturbations of the nucleus normally occur during nuclear positioning and migration. In addition, cell contraction or expansion occurring for instance during cell migration or upon changes in osmotic conditions also result innuclear mechanical stress. Recent studies in Schizosaccharomyces pombe (fission yeast) have revealed unexpected functions of cytoplasmic microtubules in nuclear architecture and chromosome behavior, and have pointed to NE-chromatin tethers as protective elements during nuclear mechanics. Here, we review and discuss how fission yeast cells can be used to understand principles underlying the dynamic interplay between genome organization and function and the effect of forces applied to the nucleus by the microtubule cytoskeleton.
PB MDPI
YR 2019
FD 2019-10-20
LK https://hdl.handle.net/10433/25195
UL https://hdl.handle.net/10433/25195
LA en
NO Cells. 2019 Oct 20;8(10):1285
NO Departamento de Biología Molecular e Ingeniería Bioquímica. Centro Andaluz de Biología del Desarrollo
DS RIO
RD May 7, 2026