%0 Journal Article
%A Álvarez Herrera, C.
%A Maisanaba, Sara
%A Repetto, Guillermo
%T Investigation of mechanisms of toxicity and exclusion by transporters of the preservatives triclosan and propylparaben using batteries of Schizosaccharomyces pombe strains
%D 2019
%U https://hdl.handle.net/10433/19707
%X Triclosan (TCS) and propylparaben (PPB) are antimicrobials widely used. They present many similarities in theirapplications and also in their human and environmental health risks. In order to investigate the mechanisms oftoxic action and the efflux pumps involved in their detoxication, we used a strategy with batteries ofSchizosaccharomyces pombe yeast strains, either defective in cell signalling, in detoxification pumps, or in cellsurveillance mechanisms. Yeast were exposed up to 20 h in solid medium or in liquid medium in 96-well plates.The mechanisms of action investigated were spindle defects (mph1), stress (pmk1), DNA interference (rad3) ordiverse effects (MDR-sup). The efflux pumps investigated were Bfr1, Pmd1, Mfs1 and Caf5 or the Pap1 transcriptionfactor. Here we show that TCS was 75 times more toxic than PPB in the wild type fission yeast. Moreoxidative stress and less protection by exclusion pumps were observed for TCS than for PPB. The cytotoxicityproduced by TCS decreased from bfr1>mfs1>pmd1 > pap1 and caf5A deficient strains. In contrast, cytotoxicconcentrations of PPB caused only a mild stress. The protection provided for PPB by the transporters was moremarked than for TCS, decreasing from Pmd1, Caf5, Mfs1 and Bfr1. Furthermore, microtubule and DNA interferenceswere revealed for PPB, according to the cytotoxicity of mph1 and rad3 defective cells, respectively. Asboth compounds present complex adverse effects at concentrations close to exposure, and their combinationclearly causes a strong potentiation, more exhaustive controls and regulations in their use should be considered.
%K Triclosan
%K Propylparaben
%K Yeast
%K Schizosaccharomyces pombe
%K Toxicity
%K Transporters
%~