RT Journal Article
T1 An Extra-Virgin Olive Oil Rich in Polyphenolic Compounds Has Antioxidant Effects in Of1 Mice.
A1 Oliveras-López, María-Jesús
A1 Berná, Genoveva
A1 Carneiro, Everardo M.
A1 López García de la Serrana, Herminia
A1 Martín, Franz
A1 López Martínez, M.Carmen
K1 Extra-Virgin Olive Oil
K1 Polyphenols
K1 Plasma antioxidant capacity
K1 Enzymatic activities
K1 Lipid peroxidation
K1 Mice
AB Extra-virgin olive oil (OO) is becoming more important in daily diets due to its beneficial effects on health, most of which are because of its antioxidant content. We studied the antioxidant activity and mechanisms of an extra-virgin OO that is rich in phenolics on pancreatic islets and liver in control mice (CTL) fed a nonpurified diet and in mice supplemented with 50 μL/d sunflower oil (SO) or 50 μL/d extra-virgin OO for 4 d. Plasma hydroxytyrosol concentration was determined by HPLC-diode array detector. Plasma antioxidant capacity, enzymatic activities, and lipid peroxidation were measured by spectrophotometry. Islet function was studied by measuring insulin release. Islet cell gene expression was examined using quantitative RT-PCR. The plasma hydroxytyrosol concentration was greater in OO mice than in CTL or SO mice (P < 0.05) and was greater in SO mice than in CTL mice. The ratio of reduced:oxidized glutathione and the antioxidant capacity in plasma was greater in OO mice than in CTL or SO mice (P < 0.05) and higher in SO mice than in CTL mice. Glucose-stimulated insulin secretion was greater in OO mice than in CTL or SO mice (P < 0.05) and was also higher in SO mice than in CTL mice. Protection against liver cell and β cell membrane lipid peroxidation was greater in OO mice than in CTL or SO mice (P < 0.05) and was greater in SO mice than in CTL mice. Catalase (CAT) expression in the islet of Langerhans was higher in OO mice than in CTL mice and SO mice (P < 0.05). The CAT and glutathione peroxidase 1 activities in the islet of Langerhans were 25% greater in OO mice than in CTL mice and higher than in SO mice (P < 0.05) and they were greater in SO mice than in CTL mice. These results indicate that, in metabolic tissues, protection by extra-virgin OO against oxidative stress occurs primarily through a direct antioxidant effect as well as through an indirect mechanism that involves greater expression and activity of certain enzymes with antioxidant activities.
PB American Society for Nutrition.
YR 2008
FD 2008-03-20
LK https://hdl.handle.net/10433/24925
UL https://hdl.handle.net/10433/24925
LA en
NO The Journal of Nutrition Volume 138, Issue 6, June 2008, Pages 1074-1078
NO Department of Molecular Biology and Biochemical Engineering, University of Pablo de Olavide of Seville, 41013 Seville, Spain
NO Andalusian Center of Molecular Biology and Regenerative Medicine, CIBERDEM
NO Department of Physiology and Biophysic, Institute of Biology, State University of Campinas, CP-13.083-970 Campinas, Brazil
NO Department of Nutrition and Food Science, Faculty of Pharmacy, University of Granada, 18071 Granada, Spain
DS RIO
RD May 6, 2026