RT Journal Article
T1 Mutagenic and genotoxic potential of pure Cylindrospermopsin by a battery of in vitro tests
A1 Puerto, María
A1 Prieto, Ana I
A1 Maisanaba, Sara
A1 Gutiérrez-Praena, Daniel
A1 Mellado-García, Pilar
A1 Jos, A
A1 Cameán, AM
K1 Cylindrospermosin
K1 Ames Test
K1 Micronucleus Test
K1 Mouse lymphoma assay
K1 Comet Assay
AB Cylindrospermopsin (CYN) is a cyanobacterial toxin with an increasing world-wide occurrence. The main routeof human exposure is through the ingestion of contaminated food and water. The European Food SafetyAuthority has identified the need to further characterize the toxicological profile of cyanotoxins and in thisregard the genotoxicity is a key toxicological effect. The data available in the scientific literature show contradictoryresults. Thus, the aim of this study was to investigate the mutagenic and genotoxic effects of pure CYNusing a battery of different in vitro assays including: the bacterial reverse-mutation assay in Salmonella typhimurium(Ames test) (0–10 μg/mL), the mammalian cell micronucleus (MN) test (0–1.35 μg/mL and 0–2 μg/mL inabsence or presence of S9 fraction, respectively) and the mouse lymphoma thymidine-kinase assay (MLA)(0–0.675 μg/mL) on L5178YTk ± cells, and the standard and enzyme-modified comet assays (0–2.5 μg/mL) onCaco-2 cells. Positive results were obtained only when the metabolic fraction S9 was employed in the MN test,suggesting pro-genotoxic properties of CYN. Also, DNA damage was not mediated by oxidative stress as CYN didnot induced changes in the modified comet assay. These data could contribute to a better risk assessment of thiscyanotoxin.
PB ELSEVIER
YR 2018
FD 2018-09-18
LK https://hdl.handle.net/10433/19832
UL https://hdl.handle.net/10433/19832
LA en
NO Food and Chemical Toxicology 121 (2018) 413–422
NO Proyectos de investigaciónMinisterio de Economía y Competitividad (AGL2015-64558-R, MINECO/FEDER, UE)
NO Area of Toxicology, Faculty of Pharmacy, Universidad de Sevilla
DS RIO
RD May 3, 2026