%0 Journal Article
%A Puerto, María
%A Prieto, Ana I
%A Maisanaba, Sara
%A Gutiérrez-Praena, Daniel
%A Mellado-García, Pilar
%A Jos, A
%A Cameán, AM
%T Mutagenic and genotoxic potential of pure Cylindrospermopsin by a battery of in vitro tests
%D 2018
%U https://hdl.handle.net/10433/19832
%X Cylindrospermopsin (CYN) is a cyanobacterial toxin with an increasing world-wide occurrence. The main routeof human exposure is through the ingestion of contaminated food and water. The European Food SafetyAuthority has identified the need to further characterize the toxicological profile of cyanotoxins and in thisregard the genotoxicity is a key toxicological effect. The data available in the scientific literature show contradictoryresults. Thus, the aim of this study was to investigate the mutagenic and genotoxic effects of pure CYNusing a battery of different in vitro assays including: the bacterial reverse-mutation assay in Salmonella typhimurium(Ames test) (0–10 μg/mL), the mammalian cell micronucleus (MN) test (0–1.35 μg/mL and 0–2 μg/mL inabsence or presence of S9 fraction, respectively) and the mouse lymphoma thymidine-kinase assay (MLA)(0–0.675 μg/mL) on L5178YTk ± cells, and the standard and enzyme-modified comet assays (0–2.5 μg/mL) onCaco-2 cells. Positive results were obtained only when the metabolic fraction S9 was employed in the MN test,suggesting pro-genotoxic properties of CYN. Also, DNA damage was not mediated by oxidative stress as CYN didnot induced changes in the modified comet assay. These data could contribute to a better risk assessment of thiscyanotoxin.
%K Cylindrospermosin
%K Ames Test
%K Micronucleus Test
%K Mouse lymphoma assay
%K Comet Assay
%~