RT Journal Article
T1 Identification of two fnr genes and characterisation of their role in the anaerobic switch in Sphingopyxis granuli strain TFA
A1 González Flores, Yolanda Elisabet
A1 Dios Barranco, Rubén de
A1 Reyes-Ramírez, Francisca
A1 Santero, Eduardo
K1 Redox regulation
K1 FNR transcriptional regulator
K1 Sphingopyxis granuli TFA
K1 Anaerobic switch
AB Sphingopyxis granuli strain TFA is able to grow on the organic solvent tetralin as the only carbon and energy source. The aerobic catabolic pathway for tetralin, the genes involved and their regulation have been fully characterised. Unlike most of the bacteria belonging to the sphingomonads group, this strain is able to grow in anoxic conditions by respiring nitrate, though not nitrite, as the alternative electron acceptor. In this work, two fnr-like genes, fnrN and fixK, have been identified in strain TFA. Both genes are functional in E. coli and Sphingopyxis granuli although fixK, whose expression is apparently activated by FnrN, seems to be much less effective than fnrN in supporting anaerobic growth. Global transcriptomic analysis of a ΔfnrN ΔfixK double mutant and identification of Fnr boxes have defined a minimal Fnr regulon in this bacterium. However, expression of a substantial number of anaerobically regulated genes was not affected in the double mutant. Additional regulators such regBA, whose expression is also activated by Fnr, might also be involved in the anaerobic response. Anaerobically induced stress response genes were not regulated by Fnr but apparently induced by stress conditions inherent to anaerobic growth, probably due to accumulation of nitrite and nitric oxide.
PB Springer Nature
YR 2020
FD 2020-12-03
LK https://hdl.handle.net/10433/25386
UL https://hdl.handle.net/10433/25386
LA en
NO Scientific Reports, volume 10, Article number: 21019 (2020)
NO This work was supported by grants BIO2014-57545-R and PGC2018-097151-B-I00, co-funded by the Spanish Ministerio de Ciencia e Innovación and the European Regional Development Fund, and by fellowships from the FPU program (Ministerio de Universidades, Spain), awarded to Yolanda Elisabet González‑Flores and Rubén de Dios.
NO Universidad Pablo de Olavide. Departamento de Biología Molecular e Ingeniería Bioquímica
DS RIO
RD Apr 23, 2026