RT Journal Article
T1 Stabilization of apoptotic cells: generation of zombie cells
A1 Oropesa Ávila, Manuel
A1 Andrade Talavera, Yuniesky
A1 Garrido Maraver, Juan
A1 Cordero, Mario
A1 Mata Fernández, Mario de la
A1 Cotan, David
A1 Villanueva Paz, Marina
A1 Delgado Pavón, Ana
A1 Alcocer Gómez, Elísabet
A1 de la Vera Calderón, Isabel
A1 Lema, Rafael
A1 Zaderenko Partida, Ana Paula
A1 Rodríguez-Moreno, Antonio
A1 Sánchez-Alcázar, José A.
K1 Apoptosis
AB Apoptosis is characterized by degradation of cell components but plasma membrane remains intact. Apoptotic microtubule network (AMN) is organized during apoptosis forming a cortical structure beneath plasma membrane that maintains plasma membrane integrity. Apoptotic cells are also characterized by high reactive oxygen species (ROS) production that can be potentially harmful for the cell. The aim of this study was to develop a method that allows stabilizing apoptotic cells for diagnostic and therapeutic applications. By using a cocktail composed of taxol (a microtubule stabilizer), Zn(2+) (a caspase inhibitor) and coenzyme Q10 (a lipid antioxidant), we were able to stabilize H460 apoptotic cells in cell cultures for at least 72 h, preventing secondary necrosis. Stabilized apoptotic cells maintain many apoptotic cell characteristics such as the presence of apoptotic microtubules, plasma membrane integrity, low intracellular calcium levels and mitochondrial polarization. Apoptotic cell stabilization may open new avenues in apoptosis detection and therapy.
PB Macmillan Publishers Limited. Nature publishing group
YR 2014
FD 2014-08-14
LK https://hdl.handle.net/10433/23688
UL https://hdl.handle.net/10433/23688
LA en
NO Cell Death and Disease (2014) 5, e1369; doi:10.1038/cddis.2014.332
NO Departamento de Fisiología, Anatomía y Biología Celular
DS RIO
RD May 26, 2026