RT Journal Article
T1 A Key Role of the EMC Complex for Mitochondrial Respiration and Quiescence in Fission Yeasts
A1 Berraquero, Modesto
A1 Álvarez Tallada, Víctor
A1 Jiménez, Juan
K1 EMC complex
K1 Ergosterol
K1 Fission yeast
K1 Mitochondria
K1 Oxygen consumption
K1 Quiescence
AB In eukaryotes, oxygen consumption is mainly driven by the respiratory activity of mitochondria, which generates most of the cellular energy that sustains life. This parameter provides direct information about mitochondrial activity of all aerobic biological systems. Using the Seahorse analyzer instrument, we show here that deletion of the oca3/emc2 gene (oca3Δ) encoding the Emc2 subunit of the ER membrane complex (EMC), a conserved chaperone/insertase that aids membrane protein biogenesis in the ER, severely affects oxygen consumption rates and quiescence survival in Schizosaccharomyces pombe yeast cells. Remarkably, the respiratory defect of the oca3Δ mutation (EMC dysfunction) is rescued synergistically by disruption of ergosterol biosynthesis (erg5Δ) and the action of the membrane fluidizing agent tween 20, suggesting a direct role of membrane fluidity and sterol composition in mitochondrial respiration in the fission yeast.
PB Wiley
YR 2025
FD 2025-04
LK https://hdl.handle.net/10433/26633
UL https://hdl.handle.net/10433/26633
LA en
NO Yeast . 2025 Apr;42(4):96-103
NO Proyectos de investigaciónPID2019‐111124GB‐I00
NO Universidad Pablo de Olavide. Departamento de Biología Molecular e Ingeniería Bioquímica
NO Centro Andaluz de Biología del Desarrollo, Consejo Superior de Investigaciones Científicas
DS RIO
RD May 23, 2026