RT Journal Article
T1 Presynaptic kainate receptor-mediated facilitation of glutamate release involves Ca2+ -calmodulin at mossy fiber-CA3 synapses
A1 Andrade Talavera, Yuniesky
A1 Duque Feria, Paloma
A1 Negrete Diaz, Jose Vicente
A1 Talvinder S, Sihra
A1 Flores Álvarez, Gonzalo
A1 Rodríguez-Moreno, Antonio
K1 Calcium calmodulin
K1 Glutamate
K1 Hippocampus
K1 Kainate receptors
K1 Presynaptic
AB Presynaptic kainate receptors (KARs) modulate the release of glutamate at synapses established between mossy fibers (MF) and CA3 pyramidal cells in the hippocampus. The activation of KAR by low, nanomolar, kainate concentrations facilitates glutamate release. KAR-mediated facilitation of glutamate release involves the activation of an adenylate cyclase/cyclic adenosine monophosphate/protein kinase A cascade at MF-CA3 synapses. Here, we studied the mechanisms by which KAR activation produces this facilitation of glutamate release in slices and synaptosomes. We find that the facilitation of glutamate release mediated by KAR activation requires an increase in Ca(2+) levels in the cytosol and the formation of a Ca(2+) -calmodulin complex to activate adenylate cyclase. The increase in cytosolic Ca(2+) underpinning this modulation is achieved, both, by Ca(2+) entering via Ca(2+) -permeable KARs and, by the mobilization of intraterminal Ca(2+) stores. Finally, we find that, congruent with the Ca(2+) -calmodulin support of KAR-mediated facilitation of glutamate release, induction of long-term potentiation at MF-CA3 synapses has an obligate requirement for Ca(2+) -calmodulin activity.
PB Wiley
YR 2012
FD 2012
LK https://hdl.handle.net/10433/23673
UL https://hdl.handle.net/10433/23673
LA en
NO Andrade-Talavera, Y., Duque-Feria, P., Negrete-Díaz, J.V., Sihra, T.S., Flores, G. and Rodríguez-Moreno, A. (2012), Presynaptic kainate receptor-mediated facilitation of glutamate release involves Ca2+–calmodulin at mossy fiber–CA3 synapses. Journal of Neurochemistry, 122: 891-899. https://doi.org/10.1111/j.1471-4159.2012.07844.x
NO Departamento de Fisiología, Anatomía y Biología Celular
DS RIO
RD May 5, 2026