RT Journal Article
T1 Protocol for the analysis of proteasome activity, assembly state, and composition in fission yeast extracts using native gels.
A1 Ruiz Romero, Gabriel
A1 Daga, Rafael
A1 Salas-Pino, Silvia
K1 Proteasome
K1 Proteasome activity
AB The proteasome is a macromolecular complex responsible for degrading short-lived or damaged proteins. Proteasome analysis presents challenges due to its high molecular weight and low stability. Here, we present a protocol to assess proteasome activity, assembly, and composition in Schizosaccharomyces pombe. We describe steps for lysate preparation and native electrophoresis to separate proteasome complexes. We then detail procedures for detecting proteasome activity using fluorescent substrates, fluorescently-tagged native proteins, and non-tagged proteasome components by immunodetection in a single gel.
PB Elsevier
YR 2025
FD 2025-12-04
LK https://hdl.handle.net/10433/25239
UL https://hdl.handle.net/10433/25239
LA en
NO STAR Protoc. 2025 Dec 4;6(4):104233. doi: 10.1016/j.xpro.2025.104233
NO MICIU/AEI/10.13039/501100011033/, FEDER UE, grant numbers PID2021-128408OB-I00 and PID2024-160582OB-I00
NO Departamento de Biología Molecular e Ingeniería Bioquímica. Centro Andaluz de Biología del Desarrollo
DS RIO
RD Apr 23, 2026