RT Journal Article
T1 Analysis of bacterial pangenomes reduces CRISPR dark matter and reveals strong association between membranome and CRISPR-Cas systems
A1 Rubio Valle, Alejandro
A1 Sprang, Maximilian
A1 Garzón, Andrés
A1 Moreno Rodriguez, Antonio
A1 Pachón Ibáñez, Maria Eugenia
A1 Pachón, Jerónimo
A1 Andrade Navarro, Miguel A.
A1 Pérez-Pulido, Antonio J.
K1 CRISPR-Cas
K1 Membrane
K1 Phage
K1 Pangenome
K1 ESKAPE
K1 Machine learning
AB CRISPR-Cas systems are prokaryotic acquired immunity mechanisms, which are found in 40% of bacterial genomes. They prevent viral infections through small DNA fragments called spacers. However, the vast majority of these spacers have not yet been associated with the virus they recognize, and it has been named CRISPR dark matter. By analyzing the spacers of tens of thousands of genomes from six bacterial species, we have been able to reduce the CRISPR dark matter from 80% to as low as 15% in some of the species. In addition, we have observed that, when a genome presents CRISPR-Cas systems, this is accompanied by particular sets of membrane proteins. Our results suggest that when bacteria present membrane proteins that make it compete better in its environment and these proteins are, in turn, receptors for specific phages, they would be forced to acquire CRISPR-Cas.
PB American Association for the Advancement of Science
YR 2023
FD 2023
LK https://hdl.handle.net/10433/25477
UL https://hdl.handle.net/10433/25477
LA en
NO Alejandro Rubio et al. ,Analysis of bacterial pangenomes reduces CRISPR dark matter and reveals strong association between membranome and CRISPR-Cas systems.Sci. Adv.9,eadd8911(2023).DOI:10.1126/sciadv.add8911
NO Universidad Pablo de Olavide. Departamento de Biología Molecular e Ingeniería Bioquímica
DS RIO
RD Apr 25, 2026