RT Journal Article
T1 Formation of Transient Protein Aggregate-like Centers Is a General Strategy Postponing Degradation of Misfolded Intermediates
A1 Boronat, S.
A1 Cabrera, M.
A1 Vega, M.
A1 Alcalá, J.
A1 Salas-Pino, Silvia
A1 Daga, Rafael
A1 Ayté, J.
A1 Hidalgo, E.
K1 Btn2
K1 Hsp104
K1 Hsp42
K1 Mas5
K1 Budding yeast
K1 Ydj1
K1 Fission yeast
K1 Heat shock response
K1 Protein aggregation-like centers
AB When misfolded intermediates accumulate during heat shock, the protein quality control system promotes cellular adaptation strategies. In Schizosaccharomyces pombe, thermo-sensitive proteins assemble upon stress into protein aggregate-like centers, PACs, to escape from degradation. The role of this protein deposition strategy has been elusive due to the use of different model systems and reporters, and to the addition of artificial inhibitors, which made interpretation of the results difficult. Here, we compare fission and budding yeast model systems, expressing the same misfolding reporters in experiments lacking proteasome or translation inhibitors. We demonstrate that mild heat shock triggers reversible PAC formation, with the collapse of both reporters and chaperones in a process largely mediated by chaperones. This assembly postpones proteasomal degradation of the misfolding reporters, and their Hsp104-dependent disassembly occurs during stress recovery. Severe heat shock induces formation of cytosolic PACs, but also of nuclear structures resembling nucleolar rings, NuRs, presumably to halt nuclear functions. Our study demonstrates that these distantly related yeasts use very similar strategies to adapt and survive to mild and severe heat shock and that aggregate-like formation is a general cellular scheme to postpone protein degradation and facilitate exit from stress.
PB MDPI
YR 2023
FD 2023-07-07
LK https://hdl.handle.net/10433/25196
UL https://hdl.handle.net/10433/25196
LA en
NO Int J Mol Sci. 2023 Jul 7;24(13):11202.
NO Departamento de Biología Molecular e Ingeniería Bioquímica. Centro Andaluz de Biología del Desarrollo
DS RIO
RD May 8, 2026