RT Journal Article
T1 Growth response of Saccharomyces cerevisiae strains to stressors associated to the vine cycle
A1 González, Magali Lucía
A1 Valero Blanco, Eva María
A1 Chimeno, Selva Valeria
A1 Garrido-Fernández, Antonio
A1 Rodríguez-Gómez, Francisco
A1 Rojo, María Cecilia
A1 Paolinelli, Marcos
A1 Arroyo-López, Francisco Noe
A1 Combina, Mariana
A1 Mercado, Laura Analía
K1 Wine yeasts
K1 Grapevine annual growth cycle
K1 Vineyard niches acclimatization
K1 Physiological variation
AB Saccharomyces cerevisiae isolates from grapes, soil, vine bark and buds collected at seven phenological stages of an annual growth cycle, were molecular typed by Microsatellite Multiplex PCR. Subsequently 30 S. cerevisiae genotypes were selected and the effect of vineyard environmental stressors, in both sublethal upper and lower levels, on their growth parameters was evaluated. The effect of low and high temperature (7–40 ◦C), pH (2.5–8.0), glucose concentration (3.0–300.0 g/L), nitrogen concentration (0.008–8.0 g/L), and copper presence (24 mg/L) were modelled individually using the reparametrized Gompertz equation. Multivariate ANOVA and Generalized Procrustes Analysis were used to determine the environmental stressor’s influence over the lag phase (λ) and the maximum specific growth rate (μmax). Both parameters were significantly affected by the S. cerevisiae genotype, the treatments, and the interaction between them. Despite a generalized reduction in μmax and a variable answer in λ, the 30 S. cerevisiae genotypes were able to overcome all the treatments. Extreme glucose limitation, copper presence and low temperature had the highest impact over the growth parameters. Interestingly, ten genotypes mostly distributed in the vineyard were the least affected, suggesting a greater acclimatization fitness and the possibility to persist in the changing conditions of the vine annual cycle.
PB Elsevier
YR 2022
FD 2022-02-05
LK https://hdl.handle.net/10433/25338
UL https://hdl.handle.net/10433/25338
LA en
NO LWT - Food Science and Technology 158 (2022) 113157
NO Universidad Pablo de Olavide. Departamento de Biología Molecular e Ingeniería Bioquímica
DS RIO
RD May 8, 2026