López-Lluch, Guillermo

Catedrático/a de Universidad
Profile Picture
First Name
Last Name
Universidad Pablo de Olavide
Fisiología, Anatomía y Biología Celular
Research Center
Centro Andaluz de Biología del Desarrollo (CABD)
Biología Celular
Research Group
Fisiología y Bioquímica del Crecimiento Celular
Biología y Biotecnología
PhD programs
Biotecnología, Biomedicina y Ciencias de la Salud, Ciencias de la Actividad Física, del Deporte y la Salud
UPO investigaORCIDScopus Author IDWeb of Science ResearcherIDDialnet IDGoogle Scholar ID

Search Results

Now showing 1 - 3 of 3
  • Publication
    Calorie Restriction Rescues Mitochondrial Dysfunction in Adck2-Deficient Skeletal Muscle
    (Frontiers Media, 2022) Hernández Camacho, Juan Diego; Moreno Fernández-Ayala, Daniel José; Vicente-García, Cristina; Navas-Enamorado, Ignacio; López-Lluch, Guillermo; Oliva, Clara; Artuch, Rafael; Garcia-Villoria, Judith; Ribes, Antonia; de Cabo, Rafael; Carvajal, Jaime; Navas, Plácido
    ADCK2 haploinsufficiency-mediated mitochondrial coenzyme Q deficiency in skeletal muscle causes mitochondrial myopathy associated with defects in beta-oxidation of fatty acids, aged-matched metabolic reprogramming, and defective physical performance. Calorie restriction has proven to increase lifespan and delay the onset of chronic diseases associated to aging. To study the possible treatment by food deprivation, heterozygous Adck2 knockout mice were fed under 40% calorie restriction (CR) and the phenotype was followed for 7 months. The overall glucose and fatty acids metabolism in muscle was restored in mutant mice to WT levels after CR. CR modulated the skeletal muscle metabolic profile of mutant mice, partially rescuing the profile of WT animals. The analysis of mitochondria isolated from skeletal muscle demonstrated that CR increased both CoQ levels and oxygen consumption rate (OCR) based on both glucose and fatty acids substrates, along with mitochondrial mass. The elevated aerobic metabolism fits with an increase of type IIa fibers, and a reduction of type IIx in mutant muscles, reaching WT levels. To further explore the effect of CR over muscle stem cells, satellite cells were isolated and induced to differentiate in culture media containing serum from animals in either ad libitum or CR diets for 72 h. Mutant cells showed slower differentiation alongside with decreased oxygen consumption. In vitro differentiation of mutant cells was increased under CR serum reaching levels of WT isolated cells, recovering respiration measured by OCR and partially beta-oxidation of fatty acids. The overall increase of skeletal muscle bioenergetics following CR intervention is paralleled with a physical activity improvement, with some increases in two and four limbs strength tests, and weights strength test. Running wheel activity was also partially improved in mutant mice under CR. These results demonstrate that CR intervention, which has been shown to improve age-associated physical and metabolic decline in WT mice, also recovers the defective aerobic metabolism and differentiation of skeletal muscle in mice caused by ADCK2 haploinsufficiency.
  • Publication
    Levels of Plasma Coenzyme Q10 Are Associated with Physical Capacity and Cardiovascular Risk in the Elderly
    (MDPI, 2022-01-29) Rocío de la Bella-Garzón; Fernández-Portero, Cristina; Alarcón, David; García Amián, Josué; López-Lluch, Guillermo
    Coenzyme Q10 (CoQ10) is an essential factor for mitochondrial activity and antioxidant protection of cells, tissues and plasma lipoproteins. Its deficiency has been associated with aging progression in animals and humans. To determine if CoQ10 levels in plasma can be associated with frailty in elderly people (aged > 65), we studied the relationship of CoQ10 levels in blood with other parameters in plasma and with the physical activity and capacity in aged people. Our results indicate that high CoQ10 levels are directly associated with lower cardiovascular risk measured by the quotient total cholesterol/HDL cholesterol. Furthermore, high CoQ10 levels were found in people showing higher physical activity, stronger muscle capacity. CoQ10 also showed a strong inverse relationship with sedentarism and the up and go test, which is considered to be a frailty index. Interestingly, we found gender differences, indicating stronger correlations in women than in men. The importance of the maintenance of CoQ10 levels in elderly people to avoid sarcopenia and frailty in elderly people is discussed.
  • Publication
    The Evolution of Physical Performance throughout an Entire Season in Female Football Players
    (MDPI, 2024) Reyes Laredo, Francisco; Pareja Blanco, Fernando; López-Lluch, Guillermo; Rodríguez-Bies, Elisabeth
    Research on the evolution of performance throughout a season in team sports is scarce and mainly focused on men’s teams. Our aim in this study was to examine the seasonal variations in relevant indices of physical performance in female football players. Twenty-seven female football players were assessed at week 2 of the season (preseason, PS), week 7 (end of preseason, EP), week 24 (half-season, HS), and week 38 (end of season, ES). Similar to the most common used conditioning tests in football, testing sessions consisted of (1) vertical countermovement jump (CMJ); (2) 20 m running sprint (T20); (3) 25 m side-step cutting maneuver test (V-CUT); and (4) progressive loading test in the full-squat exercise (V1-LOAD). Participants followed their normal football training procedure, which consisted of three weekly training sessions and an official match, without any type of intervention. No significant time effects were observed for CMJ height (p = 0.29) and T20 (p = 0.11) throughout the season. However, significant time effects were found for V-CUT (p = 0.004) and V1-LOAD (p = 0.001). V-CUT performance significantly improved from HS to ES (p = 0.001). Significant increases were observed for V1-LOAD throughout the season: PS-HS (p = 0.009); PS-ES (p < 0.001); EP-ES (p < 0.001); and HS-ES (p = 0.009). These findings suggest that, over the course of the season, female football players experience an enhancement in muscle strength and change of direction ability. However, no discernible improvements were noted in sprinting and jumping capabilities during the same period.